8PSQ

Tilapia Lake Virus polymerase in cRNA pre-initiation state mode A (core only)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Polymerase acidic protein (PA-like)	polymer	419	47780.5	1	UniProt (A0A142I7Z3) In PDB	Tilapia lake virus
2	B	Putative PB1	polymer	519	57179.4	1	UniProt (A0A1Y9SHW4) In PDB	Tilapia lake virus
3	C	RNA-dependent RNA polymerase	polymer	478	53782.1	1	UniProt (A0A7G3S745) In PDB	Tilapia lake virus
4	V, S	5' cRNA end - cRNA loop (40-mer)	polymer	40	12828.7	2		Tilapia lake virus
5	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7		water	water		18.0	1	Chemie (HOH)

Sequence modifications

C: 1 - 457 (UniProt: A0A7G3S745)

PDB	External Database	Details
Lys 391	Arg 391	conflict
Gly 458	-	expression tag
Ser 459	-	expression tag
Gly 460	-	expression tag
Ser 461	-	expression tag
Glu 462	-	expression tag
Asn 463	-	expression tag
Leu 464	-	expression tag
Tyr 465	-	expression tag
Phe 466	-	expression tag
Gln 467	-	expression tag
Gly 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	184399.4
Non-Polymers*	Number of molecules	2
	Total formula weight	89.7
All*	Total formula weight	184489.1

*Water molecules are not included.