8PSN

Tilapia Lake Virus polymerase in vRNA initiation state (transcriptase conformation)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polymerase acidic protein (PA-like)	polymer	419	47780.5	1	UniProt (A0A142I7Z3)	Tilapia lake virus
2	B (B)	Putative PB1	polymer	519	57179.4	1	UniProt (A0A1Y9SHW4)	Tilapia lake virus
3	C (C)	RNA-dependent RNA polymerase	polymer	478	53782.1	1	UniProt (A0A7G3S745)	Tilapia lake virus
4	D, E (V, S)	5' vRNA end - vRNA loop (40-mer)	polymer	40	12736.5	2		Tilapia lake virus
5	F (E)	DNA (5'-D(*(CTP))-3')	polymer	1	483.2	1		synthetic construct
6	G, I, J (A, C)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
7	H, K (B, E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	L, M (A, B)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

C: 1 - 457 (UniProt: A0A7G3S745)

PDB	External Database	Details
Lys 391	Arg 391	conflict
Gly 458	-	expression tag
Ser 459	-	expression tag
Gly 460	-	expression tag
Ser 461	-	expression tag
Glu 462	-	expression tag
Asn 463	-	expression tag
Leu 464	-	expression tag
Tyr 465	-	expression tag
Phe 466	-	expression tag
Gln 467	-	expression tag
Gly 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	184698.2
Non-Polymers*	Number of molecules	5
	Total formula weight	244.8
All*	Total formula weight	184943.1

*Water molecules are not included.