8PR7

Aurora-A in complex with CEP192 and an inhibitory monobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Aurora kinase A	polymer	283	32581.4	2	UniProt (O14965) Pfam (PF00069)	Homo sapiens (human)	Aurora 2,Aurora/IPL1-related kinase 1,ARK-1,Aurora-related kinase 1,Breast tumor-amplified kinase,Ipl1- and aurora-related kinase 1,Serine/threonine-protein kinase 15,Serine/threonine-protein kinase 6,Serine/threonine-protein kinase Ayk1,Serine/threonine-protein kinase aurora-A
2	B, E (B, E)	Monobody	polymer	96	10129.3	2		synthetic construct
3	C, F (C, F)	Centrosomal protein of 192 kDa	polymer	69	8049.6	2	UniProt (Q8TEP8)	Homo sapiens (human)	Cep192,Cep192/SPD-2
4	G, H (A, D)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
5	I (D)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	J (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	K, L, M, N, O (A, B, C, D, E)	water	water		18.0	87	Chemie (HOH)

Sequence modifications

A, D: 122 - 403 (UniProt: O14965)

PDB	External Database	Details
Met 121	-	initiating methionine
Asn 274	Asp 274	engineered mutation
Ala 290	Cys 290	engineered mutation
Ala 332	Asn 332	engineered mutation
Ala 335	Gln 335	engineered mutation
Ala 347	Thr 347	engineered mutation
Ala 350	Asp 350	engineered mutation
Ala 393	Cys 393	engineered mutation

C, F: 468 - 533 (UniProt: Q8TEP8)

PDB	External Database	Details
Gly 465	-	expression tag
Ser 466	-	expression tag
Met 467	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	101520.6
Non-Polymers*	Number of molecules	4
	Total formula weight	970.8
All*	Total formula weight	102491.4

*Water molecules are not included.