8PP3

Binary crystal structure of positively supercharged ferritin variant Ftn(pos) and crystal contact tuned negatively supercharged ferritin variant Ftn(neg)-m1 (Mg formate condition)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Ferritin heavy chain	polymer	183	21499.2	6	UniProt (P02794) Pfam (PF00210) In PDB	Homo sapiens (human)	Ferritin H subunit,Cell proliferation-inducing gene 15 protein
2	G, H, I, J, K...	Ferritin heavy chain	polymer	183	21297.5	6	UniProt (P02794) Pfam (PF00210) In PDB	Homo sapiens (human)	Ferritin H subunit,Cell proliferation-inducing gene 15 protein
3	J, K, L, A, B...	FE (III) ION	non-polymer		55.8	12	Chemie (FE)
4	B, D, F	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	J, H	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6		water	water		18.0	976	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 0 - 182 (UniProt: P02794)

PDB	External Database	Details
Lys 18	Ala 19	engineered mutation
Arg 25	Asn 26	engineered mutation
Gln 86	Lys 87	engineered mutation
Lys 90	Cys 91	engineered mutation
Arg 98	Asn 99	engineered mutation
Lys 102	Cys 103	engineered mutation
Lys 105	His 106	engineered mutation
Lys 109	Asn 110	engineered mutation
Lys 123	Asp 124	engineered mutation
Arg 162	Glu 163	engineered mutation

G, H, I, J, K, L: 0 - 182 (UniProt: P02794)

PDB	External Database	Details
Gln 86	Lys 87	engineered mutation
Glu 90	Cys 91	engineered mutation
Glu 102	Cys 103	engineered mutation
Glu 105	His 106	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	256780.6
Non-Polymers*	Number of molecules	18
	Total formula weight	1087.1
All*	Total formula weight	257867.7

*Water molecules are not included.