8PMS
NADase from Aspergillus fumigatus with replaced C-terminus from Neurospora crassa
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Conidial surface nicotinamide adenine dinucleotide glycohydrolase nadA | polymer | 239 | 26651.1 | 4 | UniProt (Q4WL81) Pfam (PF14021) | Aspergillus fumigatus Af293 | NADase,NAD(+) hydrolase nadA,NADP(+) hydrolase nadA |
| 2 | E (E) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 570.5 | 1 | In PDB GlyTouCan (G21290RB) | |||
| 3 | F (F) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 894.8 | 1 | In PDB GlyTouCan (G42466VF) | |||
| 4 | G (G) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 5 | H, I, N, O, T... (A, B, C, D) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 9 | Chemie (NAG) | |||
| 6 | J, P, Q (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 7 | K, R (A, B) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 2 | Chemie (EPE) | |||
| 8 | L, M, S, V (A, B, C) | ACRYLIC ACID | non-polymer | 72.1 | 4 | Chemie (AKR) | |||
| 9 | AA, BA, CA, Z (B, C, D, A) | water | water | 18.0 | 134 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 223 (UniProt: Q4WL81)
| PDB | External Database | Details |
|---|---|---|
| Glu 213 | Leu 213 | engineered mutation |
| Pro 215 | Glu 215 | engineered mutation |
| Gln 216 | Ser 216 | engineered mutation |
| Arg 217 | Glu 217 | engineered mutation |
| Leu 218 | Tyr 218 | engineered mutation |
| Val 219 | Asp 219 | engineered mutation |
| Pro 220 | Glu 220 | engineered mutation |
| Arg 221 | Lys 221 | engineered mutation |
| Asn 222 | Val 222 | engineered mutation |
| Tyr 223 | Glu 223 | engineered mutation |
| Gly 224 | - | expression tag |
| Thr 225 | - | expression tag |
| Asp 226 | - | expression tag |
| Val 227 | - | expression tag |
| Leu 228 | - | expression tag |
| Phe 229 | - | expression tag |
| Gln 230 | - | expression tag |
| Gly 231 | - | expression tag |
| Pro 232 | - | expression tag |
| Gly 233 | - | expression tag |
| His 234 | - | expression tag |
| His 235 | - | expression tag |
| His 236 | - | expression tag |
| His 237 | - | expression tag |
| His 238 | - | expression tag |
| His 239 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 106604.6 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1889.8 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 3032.0 | |
| All* | Total formula weight | 111526.4 |
