8PM0

Influenza A/H7N9 polymerase in replicase-like conformation in pre-initiation state with Pol II pS5 CTD peptide mimic bound in site 1A/2A

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Polymerase acidic protein	polymer	729	84354.2	1	UniProt (M9TI86) Pfam (PF00603) In PDB	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
2	B	RNA-directed RNA polymerase catalytic subunit	polymer	757	86496.2	1	UniProt (M9TLW3) Pfam (PF00602) In PDB	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))	Polymerase basic protein 1,PB1,RNA-directed RNA polymerase subunit P1
3	C	Polymerase basic protein 2	polymer	788	89037.6	1	UniProt (X5F427) Pfam (PF20947) Pfam (PF20948) Pfam (PF20949) Pfam (PF20950) Pfam (PF00604) Pfam (PF20951) Pfam (PF20952) In PDB	Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
4	V	51-mer vRNA loop (v51_mut_S)	polymer	51	16093.4	1		Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
5	P, G	RNA Pol II CTD 6 repeats (site 1A/2A)	polymer	42	4816.3	2		Homo sapiens
6	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 716 (UniProt: M9TI86)

PDB	External Database	Details
Met -12	-	initiating methionine
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Lys 349	Glu 349	engineered mutation
Ile 490	Arg 490	engineered mutation

C: 1 - 759 (UniProt: X5F427)

PDB	External Database	Details
Arg 74	Gly 74	engineered mutation
Gly 760	-	expression tag
Trp 761	-	expression tag
Ser 762	-	expression tag
His 763	-	expression tag
Pro 764	-	expression tag
Gln 765	-	expression tag
Phe 766	-	expression tag
Glu 767	-	expression tag
Lys 768	-	expression tag
Gly 769	-	expression tag
Gly 770	-	expression tag
Gly 771	-	expression tag
Ser 772	-	expression tag
Gly 773	-	expression tag
Gly 774	-	expression tag
Gly 775	-	expression tag
Ser 776	-	expression tag
Gly 777	-	expression tag
Gly 778	-	expression tag
Ser 779	-	expression tag
Ala 780	-	expression tag
Trp 781	-	expression tag
Ser 782	-	expression tag
His 783	-	expression tag
Pro 784	-	expression tag
Gln 785	-	expression tag
Phe 786	-	expression tag
Glu 787	-	expression tag
Lys 788	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	285614.0
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	285638.3

*Water molecules are not included.