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8PHK

fully recruited RfaH bound to E. coli transcription complex paused at ops site

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(P)
Transcription antitermination protein RfaHpolymer16418559.41UniProt (P0AFW0)
Pfam (PF02357)
Escherichia coli
2B
(I)
DNA-directed RNA polymerase subunit betapolymer1342150820.91UniProt (P0A8V2)
Pfam (PF04560)
Pfam (PF04563)
Pfam (PF04561)
Pfam (PF04565)
Pfam (PF10385)
Pfam (PF00562)
Escherichia coliRNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3C
(J)
DNA-directed RNA polymerase subunit beta'polymer1416156505.01UniProt (P0A8T7)
Pfam (PF04997)
Pfam (PF00623)
Pfam (PF04983)
Pfam (PF05000)
Pfam (PF04998)
Escherichia coliRNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4D
(K)
DNA-directed RNA polymerase subunit omegapolymer9110249.51UniProt (P0A800)
Pfam (PF01192)
Escherichia coliRNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5E, F
(G, H)
DNA-directed RNA polymerase subunit alphapolymer32936558.72UniProt (P0A7Z4)
Pfam (PF01193)
Pfam (PF01000)
Pfam (PF03118)
Escherichia coliRNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
6G
(A)
non-template DNApolymer4012207.81Escherichia coli
7H
(B)
template DNApolymer3912092.81Escherichia coli
8I
(R)
RNApolymer175414.21Escherichia coli
9J, K
(J)
ZINC IONnon-polymer65.42Chemie (ZN)
10L
(J)
MAGNESIUM IONnon-polymer24.31Chemie (MG)
Sequence modifications
P: 1 - 162 (UniProt: P0AFW0)
PDBExternal DatabaseDetails
Gly -1-expression tag
His 0-expression tag
J: 2 - 1407 (UniProt: P0A8T7)
PDBExternal DatabaseDetails
Val 1-expression tag
Leu 1408-expression tag
Glu 1409-expression tag
Val 1410-expression tag
His 1411-expression tag
His 1412-expression tag
His 1413-expression tag
His 1414-expression tag
His 1415-expression tag
His 1416-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains9
Total formula weight438966.9
Non-Polymers*Number of molecules3
Total formula weight155.1
All*Total formula weight439122.1
*Water molecules are not included.

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PDB entries from 2024-11-06

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