Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8PHJ

cA4-bound Cami1 in complex with 70S ribosome

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (0)	Large ribosomal subunit protein bL33	polymer	55	6388.6	1	UniProt (P0A7N9) Pfam (PF00471)	Escherichia coli	50S ribosomal protein L33
2	B (1)	Large ribosomal subunit protein bL34	polymer	46	5397.5	1	UniProt (P0A7P5) Pfam (PF00468)	Escherichia coli	50S ribosomal protein L34
3	C (2)	Large ribosomal subunit protein bL35	polymer	65	7313.0	1	UniProt (P0A7Q1) Pfam (PF01632)	Escherichia coli	50S ribosomal protein L35,Ribosomal protein A
4	D (3)	Large ribosomal subunit protein bL36A	polymer	38	4377.4	1	UniProt (P0A7Q6) Pfam (PF00444)	Escherichia coli	50S ribosomal protein L36,Ribosomal protein B
5	E (4)	Large ribosomal subunit protein bL31A	polymer	70	7887.1	1	UniProt (P0A7M9) Pfam (PF01197)	Escherichia coli	50S ribosomal protein L31
6	F (5)	Large ribosomal subunit protein uL10	polymer	165	17736.6	1	UniProt (P0A7J3) Pfam (PF00466)	Escherichia coli	50S ribosomal protein L10,50S ribosomal protein L8
7	G (6)	Large ribosomal subunit protein uL11	polymer	142	14894.4	1	UniProt (P0A7J7) Pfam (PF03946) Pfam (PF00298)	Escherichia coli	50S ribosomal protein L11
8	H, I (7, 8)	fMet-tRNA(fMet)	polymer	77	24846.9	2	GenBank (1220402399)	Escherichia coli
9	J (9)	mRNA	polymer	31	10192.3	1		synthetic construct
10	K (A)	16S rRNA	polymer	1542	499873.4	1	GenBank (1758835854)	Escherichia coli
11	L (B)	Small ribosomal subunit protein uS2	polymer	241	26781.7	1	UniProt (P0A7V0) Pfam (PF00318)	Escherichia coli	30S ribosomal protein S2
12	M (C)	Small ribosomal subunit protein uS3	polymer	233	26031.3	1	UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189)	Escherichia coli	30S ribosomal protein S3
13	N (D)	Small ribosomal subunit protein uS4	polymer	206	23514.2	1	UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479)	Escherichia coli	30S ribosomal protein S4
14	O (E)	Small ribosomal subunit protein uS5	polymer	167	17629.4	1	UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719)	Escherichia coli	30S ribosomal protein S5
15	P (F)	Small ribosomal subunit protein bS6, fully modified isoform	polymer	135	15727.5	1	UniProt (P02358) Pfam (PF01250)	Escherichia coli
16	Q (G)	Small ribosomal subunit protein uS7	polymer	179	20055.2	1	UniProt (P02359) Pfam (PF00177)	Escherichia coli	30S ribosomal protein S7
17	R (H)	Small ribosomal subunit protein uS8	polymer	130	14146.6	1	UniProt (P0A7W7) Pfam (PF00410)	Escherichia coli	30S ribosomal protein S8
18	S (I)	Small ribosomal subunit protein uS9	polymer	130	14886.3	1	UniProt (P0A7X3) Pfam (PF00380)	Escherichia coli	30S ribosomal protein S9
19	T (J)	Small ribosomal subunit protein uS10	polymer	103	11755.6	1	UniProt (P0A7R5) Pfam (PF00338)	Escherichia coli	30S ribosomal protein S10,Transcription termination/antitermination protein NusE
20	U (K)	Small ribosomal subunit protein uS11	polymer	129	13872.0	1	UniProt (P0A7R9) Pfam (PF00411)	Escherichia coli	30S ribosomal protein S11
21	V (L)	Small ribosomal subunit protein uS12	polymer	124	13768.2	1	UniProt (P0A7S3) Pfam (PF00164)	Escherichia coli	30S ribosomal protein S12
22	W (M)	Small ribosomal subunit protein uS13	polymer	118	13128.5	1	UniProt (P0A7S9) Pfam (PF00416)	Escherichia coli	30S ribosomal protein S13
23	X (N)	Small ribosomal subunit protein uS14	polymer	101	11606.6	1	UniProt (P0AG59) Pfam (PF00253)	Escherichia coli	30S ribosomal protein S14
24	Y (O)	Small ribosomal subunit protein uS15	polymer	89	10290.8	1	UniProt (P0ADZ4) Pfam (PF00312)	Escherichia coli	30S ribosomal protein S15
25	Z (P)	Small ribosomal subunit protein bS16	polymer	82	9207.6	1	UniProt (P0A7T3) Pfam (PF00886)	Escherichia coli	30S ribosomal protein S16
26	AA (Q)	Small ribosomal subunit protein uS17	polymer	84	9724.5	1	UniProt (P0AG63) Pfam (PF00366)	Escherichia coli	30S ribosomal protein S17
27	BA (R)	Small ribosomal subunit protein bS18	polymer	75	9005.5	1	UniProt (P0A7T7) Pfam (PF01084)	Escherichia coli	30S ribosomal protein S18
28	CA (S)	Small ribosomal subunit protein uS19	polymer	92	10455.4	1	UniProt (P0A7U3) Pfam (PF00203)	Escherichia coli	30S ribosomal protein S19
29	DA (T)	Small ribosomal subunit protein bS20	polymer	87	9708.5	1	UniProt (P0A7U7) Pfam (PF01649)	Escherichia coli	30S ribosomal protein S20
30	EA (U)	Small ribosomal subunit protein bS21	polymer	71	8524.0	1	UniProt (P68679) Pfam (PF01165)	Escherichia coli	30S ribosomal protein S21
31	FA (W)	Large ribosomal subunit protein bL12	polymer	120	12178.0	1	UniProt (P0A7K2) Pfam (PF16320) Pfam (PF00542)	Escherichia coli	50S ribosomal protein L7/L12,L8
32	GA, HA (X, Y)	CRISPR-associated protein, APE2256 family	polymer	406	45821.1	2	UniProt (D3RW14) Pfam (PF09651)	Allochromatium vinosum	Cami1,CRISPR-associated protein Cami1
33	IA (a)	23S rRNA (2862-MER)	polymer	2904	941811.6	1		Escherichia coli
34	JA (b)	5S rRNA	polymer	120	38790.1	1	GenBank (CP035706.1)	Escherichia coli
35	KA (c)	Large ribosomal subunit protein uL2	polymer	273	29923.6	1	UniProt (P60422) Pfam (PF00181) Pfam (PF03947)	Escherichia coli	50S ribosomal protein L2
36	LA (d)	Large ribosomal subunit protein uL3	polymer	209	22291.6	1	UniProt (P60438) Pfam (PF00297)	Escherichia coli	50S ribosomal protein L3
37	MA (e)	Large ribosomal subunit protein uL4	polymer	201	22121.6	1	UniProt (P60723) Pfam (PF00573)	Escherichia coli	50S ribosomal protein L4
38	NA (f)	Large ribosomal subunit protein uL5	polymer	179	20333.6	1	UniProt (P62399) Pfam (PF00281) Pfam (PF00673)	Escherichia coli	50S ribosomal protein L5
39	OA (g)	Large ribosomal subunit protein uL6	polymer	177	18932.8	1	UniProt (P0AG55) Pfam (PF00347)	Escherichia coli	50S ribosomal protein L6
40	PA (h)	Large ribosomal subunit protein bL9	polymer	149	15789.0	1	UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948)	Escherichia coli	50S ribosomal protein L9
41	QA (i)	Large ribosomal subunit protein uL13	polymer	142	16050.6	1	UniProt (P0AA10) Pfam (PF00572)	Escherichia coli	50S ribosomal protein L13
42	RA (j)	Large ribosomal subunit protein uL14	polymer	123	13565.1	1	UniProt (P0ADY3) Pfam (PF00238)	Escherichia coli	50S ribosomal protein L14
43	SA (k)	Large ribosomal subunit protein uL15	polymer	144	15008.5	1	UniProt (P02413) Pfam (PF00828)	Escherichia coli	50S ribosomal protein L15
44	TA (l)	Large ribosomal subunit protein uL16	polymer	136	15312.3	1	UniProt (P0ADY7) Pfam (PF00252)	Escherichia coli	50S ribosomal protein L16
45	UA (m)	Large ribosomal subunit protein bL17	polymer	127	14393.7	1	UniProt (P0AG44) Pfam (PF01196)	Escherichia coli	50S ribosomal protein L17
46	VA (n)	Large ribosomal subunit protein uL18	polymer	117	12794.7	1	UniProt (P0C018) Pfam (PF00861)	Escherichia coli	50S ribosomal protein L18
47	WA (o)	Large ribosomal subunit protein bL19	polymer	115	13159.3	1	UniProt (P0A7K6) Pfam (PF01245)	Escherichia coli	50S ribosomal protein L19
48	XA (p)	Large ribosomal subunit protein bL20	polymer	118	13528.0	1	UniProt (P0A7L3) Pfam (PF00453)	Escherichia coli	50S ribosomal protein L20
49	YA (q)	Large ribosomal subunit protein bL21	polymer	103	11586.4	1	UniProt (P0AG48) Pfam (PF00829)	Escherichia coli	50S ribosomal protein L21
50	ZA (r)	Large ribosomal subunit protein uL22	polymer	110	12253.4	1	UniProt (P61175) Pfam (PF00237)	Escherichia coli	50S ribosomal protein L22
51	AB (s)	Large ribosomal subunit protein uL23	polymer	100	11222.2	1	UniProt (P0ADZ0) Pfam (PF00276)	Escherichia coli	50S ribosomal protein L23
52	BB (t)	Large ribosomal subunit protein uL24	polymer	104	11339.3	1	UniProt (P60624) Pfam (PF00467) Pfam (PF17136)	Escherichia coli	50S ribosomal protein L24
53	CB (u)	Large ribosomal subunit protein bL25	polymer	94	10713.5	1	UniProt (P68919) Pfam (PF01386)	Escherichia coli	50S ribosomal protein L25
54	DB (v)	Large ribosomal subunit protein bL27	polymer	85	9146.5	1	UniProt (P0A7L8) Pfam (PF01016)	Escherichia coli	50S ribosomal protein L27
55	EB (w)	Large ribosomal subunit protein bL28	polymer	78	9027.6	1	UniProt (P0A7M2) Pfam (PF00830)	Escherichia coli	50S ribosomal protein L28
56	FB (x)	Large ribosomal subunit protein uL29	polymer	63	7286.5	1	UniProt (P0A7M6) Pfam (PF00831)	Escherichia coli	50S ribosomal protein L29
57	GB (y)	Large ribosomal subunit protein uL30	polymer	59	6554.8	1	UniProt (P0AG51) Pfam (PF00327)	Escherichia coli	50S ribosomal protein L30
58	HB (z)	Large ribosomal subunit protein bL32	polymer	57	6463.4	1	UniProt (P0A7N4) Pfam (PF01783)	Escherichia coli	50S ribosomal protein L32
59	IB (Z)	Cyclic tetraadenosine monophosphate (cA4)	polymer	4	1271.9	1	BIRD (PRD_002431)	Streptococcus thermophilus
60	JB, KB (3, 4)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
61	AC, AD, AE, AF, AG... (A, a, b, c, z)	MAGNESIUM ION	non-polymer		24.3	241	Chemie (MG)

Sequence modifications

K: 1 - 129 (UniProt: P0A7R9)

PDB	External Database	Details
Ias 119	Asn 119	modified residue

X, Y: 1 - 381 (UniProt: D3RW14)

PDB	External Database	Details
Ala 2	Pro 2	engineered mutation
Ala 11	Ser 11	engineered mutation
Ala 343	His 343	engineered mutation
Gly 382	-	expression tag
Glu 383	-	expression tag
Gly 384	-	expression tag
Trp 385	-	expression tag
Ser 386	-	expression tag
His 387	-	expression tag
Pro 388	-	expression tag
Gln 389	-	expression tag
Phe 390	-	expression tag
Glu 391	-	expression tag
Lys 392	-	expression tag
Gly 393	-	expression tag
Val 394	-	expression tag
Glu 395	-	expression tag
Gly 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag

Sequence viewer

Contents of the asymmetric unit

Polymers	Number of chains	61
Polymers	Total formula weight	2338064.4
Non-Polymers*	Number of molecules	243
Non-Polymers*	Total formula weight	5988.3
All*	Total formula weight	2344052.7

*Water molecules are not included.

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)