8PH9

E. coli RNA polymerase paused at ops site (non-complementary scaffold)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	G, H	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	J	DNA-directed RNA polymerase subunit beta'	polymer	1416	156505.0	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	R	RNA	polymer	17	5382.2	1		Escherichia coli
6	A	non-template DNA	polymer	40	12207.8	1		Escherichia coli
7	B	template DNA	polymer	40	12446.0	1		Escherichia coli
8	J	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
9	J	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

J: 2 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Val 1	-	expression tag
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Val 1410	-	expression tag
His 1411	-	expression tag
His 1412	-	expression tag
His 1413	-	expression tag
His 1414	-	expression tag
His 1415	-	expression tag
His 1416	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	420728.7
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	420883.9

*Water molecules are not included.