8PFA

X-ray structure of the Thermus thermophilus K167R mutant of the PilF-GSPIIB domain in the c-di-GMP bound state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	c-di-GMP binding domain of the ATPase enzyme PilF	polymer	146	16297.6	2	UniProt (Q72H73) In PDB	Thermus thermophilus HB27	c-di-GMP binding domain of the ATPase enzyme PilF
2	A, B	9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)	non-polymer		690.4	2	Chemie (C2E)
3	A, B	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
4	A, B	ACETATE ION	non-polymer		59.0	5	Chemie (ACT)
5		water	water		18.0	96	Chemie (HOH)

Sequence modifications

A, B: 159 - 302 (UniProt: Q72H73)

PDB	External Database	Details
Gly 157	-	expression tag
Ser 158	-	expression tag
Arg 167	Lys 167	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	32595.2
Non-Polymers*	Number of molecules	17
	Total formula weight	2636.7
All*	Total formula weight	35231.8

*Water molecules are not included.