8PBL
E. coli RNA polymerase elongation complex stalled at thymine dimer lesion
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Non-template DNA | polymer | 49 | 15107.7 | 1 | Escherichia coli | ||
2 | B | Template DNA | polymer | 49 | 15384.8 | 1 | Escherichia coli | ||
3 | D, E | DNA-directed RNA polymerase subunit alpha | polymer | 239 | 26487.2 | 2 | UniProt (A0A5B9AW69) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) In PDB | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
4 | F | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) In PDB | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
5 | G | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
6 | H | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
7 | R | mRNA | polymer | 19 | 6149.7 | 1 | Escherichia coli | ||
8 | G | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
9 | R | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
D, E: 1 - 235 (UniProt: A0A5B9AW69)
PDB | External Database | Details |
---|---|---|
Val 236 | - | expression tag |
Leu 237 | - | expression tag |
Phe 238 | - | expression tag |
Gln 239 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 406053.9 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 155.1 | |
All* | Total formula weight | 406209.0 |