8P8G

Nitrogenase MoFe protein from A. vinelandii beta double mutant D353G/D357G

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Nitrogenase protein alpha chain	polymer	500	56467.2	2	UniProt (C1DGZ7) Pfam (PF00148) UniProt (by SIFTS) (P07328)	Azotobacter vinelandii DJ
2	B, D (B, D)	Nitrogenase molybdenum-iron protein beta chain	polymer	523	59419.8	2	UniProt (C1DGZ8) Pfam (PF11844) Pfam (PF00148) UniProt (by SIFTS) (P07329)	Azotobacter vinelandii DJ	Dinitrogenase
3	CA, DA, E, EA, FA... (C, A, D, B)	1,2-ETHANEDIOL	non-polymer		62.1	35	Chemie (EDO)
4	F, Y (A, C)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	AA, G (C, A)	3-HYDROXY-3-CARBOXY-ADIPIC ACID	non-polymer		206.2	2	Chemie (HCA)
6	BA, H (C, A)	iron-sulfur-molybdenum cluster with interstitial carbon	non-polymer		787.5	2	Chemie (ICS)
7	R, UA (B, D)	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	2	Chemie (DIO)
8	U (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
9	W, ZA (B, D)	FE(8)-S(7) CLUSTER, OXIDIZED	non-polymer		671.2	2	Chemie (1CL)
10	AB, X (D, B)	FE(8)-S(7) CLUSTER	non-polymer		671.2	2	Chemie (CLF)
11	Z (C)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
12	BB, CB, DB, EB (A, B, C, D)	water	water		18.0	2173	Chemie (HOH)

Sequence modifications

A, C: 3 - 492 (UniProt: C1DGZ7)

PDB	External Database	Details
Met -7	-	initiating methionine
Thr -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag

B, D: 1 - 523 (UniProt: C1DGZ8)

PDB	External Database	Details
Gly 353	Asp 353	engineered mutation
Gly 357	Asp 357	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	231774.1
Non-Polymers*	Number of molecules	49
	Total formula weight	7206.6
All*	Total formula weight	238980.7

*Water molecules are not included.