8P8G
Nitrogenase MoFe protein from A. vinelandii beta double mutant D353G/D357G
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | Nitrogenase protein alpha chain | polymer | 500 | 56467.2 | 2 | UniProt (C1DGZ7) Pfam (PF00148) UniProt (by SIFTS) (P07328) In PDB | Azotobacter vinelandii DJ | |
2 | B, D | Nitrogenase molybdenum-iron protein beta chain | polymer | 523 | 59419.8 | 2 | UniProt (C1DGZ8) Pfam (PF11844) Pfam (PF00148) UniProt (by SIFTS) (P07329) In PDB | Azotobacter vinelandii DJ | Dinitrogenase |
3 | C, A, D, B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 35 | Chemie (EDO) | |||
4 | A, C | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
5 | C, A | 3-HYDROXY-3-CARBOXY-ADIPIC ACID | non-polymer | 206.2 | 2 | Chemie (HCA) | |||
6 | C, A | iron-sulfur-molybdenum cluster with interstitial carbon | non-polymer | 787.5 | 2 | Chemie (ICS) | |||
7 | B, D | 1,4-DIETHYLENE DIOXIDE | non-polymer | 88.1 | 2 | Chemie (DIO) | |||
8 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
9 | B, D | FE(8)-S(7) CLUSTER, OXIDIZED | non-polymer | 671.2 | 2 | Chemie (1CL) | |||
10 | D, B | FE(8)-S(7) CLUSTER | non-polymer | 671.2 | 2 | Chemie (CLF) | |||
11 | C | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
12 | water | water | 18.0 | 2173 | Chemie (HOH) |
Sequence modifications
A, C: 3 - 492 (UniProt: C1DGZ7)
B, D: 1 - 523 (UniProt: C1DGZ8)
PDB | External Database | Details |
---|---|---|
Met -7 | - | initiating methionine |
Thr -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
His 1 | - | expression tag |
His 2 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly 353 | Asp 353 | engineered mutation |
Gly 357 | Asp 357 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 231774.1 | |
Non-Polymers* | Number of molecules | 49 |
Total formula weight | 7206.6 | |
All* | Total formula weight | 238980.7 |