8P73
Cryo-EM structure of CAK in complex with inhibitor ICEC0829
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | H | CDK-activating kinase assembly factor MAT1 | polymer | 93 | 10234.5 | 1 | UniProt (P51948) In PDB | Homo sapiens (human) | CDK7/cyclin-H assembly factor,Cyclin-G1-interacting protein,Menage a trois,RING finger protein 66,RING finger protein MAT1,p35,p36 |
2 | I | Cyclin-H | polymer | 324 | 37721.5 | 1 | UniProt (P51946) Pfam (PF00134) Pfam (PF16899) In PDB | Homo sapiens (human) | MO15-associated protein,p34,p37 |
3 | J | Cyclin-dependent kinase 7 | polymer | 349 | 39362.6 | 1 | UniProt (P50613) Pfam (PF00069) In PDB | Homo sapiens (human) | 39 kDa protein kinase,p39 Mo15,CDK-activating kinase 1,Cell division protein kinase 7,Serine/threonine-protein kinase 1,TFIIH basal transcription factor complex kinase subunit |
4 | J | (3R,4R)-4-[[[7-[(phenylmethyl)amino]-3-propan-2-yl-pyrazolo[1,5-a]pyrimidin-5-yl]amino]methyl]piperidine-3,4-diol | non-polymer | 410.5 | 1 | Chemie (X50) | |||
5 | J | (3S,4S)-4-[[[7-[(phenylmethyl)amino]-3-propan-2-yl-pyrazolo[1,5-a]pyrimidin-5-yl]amino]methyl]piperidine-3,4-diol | non-polymer | 410.5 | 1 | Chemie (X58) | |||
6 | water | water | 18.0 | 67 | Chemie (HOH) |
Sequence modifications
H: 220 - 309 (UniProt: P51948)
I: 1 - 323 (UniProt: P51946)
J: 1 - 346 (UniProt: P50613)
PDB | External Database | Details |
---|---|---|
Ser 217 | - | expression tag |
Asn 218 | - | expression tag |
Ala 219 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ace 0 | - | acetylation |
PDB | External Database | Details |
---|---|---|
Ser -2 | - | expression tag |
Asn -1 | - | expression tag |
Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 87318.6 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 821.0 | |
All* | Total formula weight | 88139.7 |