8P6O
Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant Y220F in complex with di-mannuronic acid
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Alginate lyase | polymer | 234 | 25528.2 | 1 | UniProt (A0A7I9C8Z1) Pfam (PF08787) In PDB | Paradendryphiella salina | |
2 | B | beta-D-mannopyranuronic acid-(1-4)-alpha-D-mannopyranuronic acid | branched | 370.3 | 1 | In PDB GlyTouCan (G11270VO) | |||
3 | C | beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid | branched | 370.3 | 1 | In PDB GlyTouCan (G06440CO) | |||
4 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
5 | water | water | 18.0 | 377 | Chemie (HOH) |
Sequence modifications
A: 17 - 240 (UniProt: A0A7I9C8Z1)
PDB | External Database | Details |
---|---|---|
Glu -1 | - | expression tag |
Phe 0 | - | expression tag |
Phe 220 | Tyr 220 | engineered mutation |
Val 241 | - | expression tag |
Asp 242 | - | expression tag |
His 243 | - | expression tag |
His 244 | - | expression tag |
His 245 | - | expression tag |
His 246 | - | expression tag |
His 247 | - | expression tag |
His 248 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 25528.2 | |
Branched | Number of molecules | 2 |
Total formula weight | 740.5 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 96.1 | |
All* | Total formula weight | 26364.8 |