8P5Y
Artificial transfer hydrogenase with a Mn-12 cofactor and Streptavidin S112Y-K121M mutant
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Streptavidin | polymer | 159 | 16647.1 | 4 | UniProt (P22629) Pfam (PF01382) In PDB | Streptomyces avidinii | |
2 | A, B, C, D | 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-(3,4-dihydro-2~{H}-pyrano[2,3-c]pyridin-6-ylmethylamino)ethyl]pentanamide | non-polymer | 433.6 | 4 | Chemie (WZQ) | |||
3 | A, B | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
4 | A, C, D | BROMIDE ION | non-polymer | 79.9 | 4 | Chemie (BR) | |||
5 | D | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
6 | water | water | 18.0 | 94 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 15 - 159 (UniProt: P22629)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Ala 2 | - | expression tag |
Ser 3 | - | expression tag |
Met 4 | - | expression tag |
Thr 5 | - | expression tag |
Gly 6 | - | expression tag |
Gly 7 | - | expression tag |
Gln 8 | - | expression tag |
Gln 9 | - | expression tag |
Met 10 | - | expression tag |
Gly 11 | - | expression tag |
Arg 12 | - | expression tag |
Asp 13 | - | expression tag |
Gln 14 | - | expression tag |
Tyr 112 | Ser 136 | engineered mutation |
Met 121 | Lys 145 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 66588.6 | |
Non-Polymers* | Number of molecules | 13 |
Total formula weight | 2309.1 | |
All* | Total formula weight | 68897.7 |