8P5Y
Artificial transfer hydrogenase with a Mn-12 cofactor and Streptavidin S112Y-K121M mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Streptavidin | polymer | 159 | 16647.1 | 4 | UniProt (P22629) | Streptomyces avidinii | |
| 2 | E, I, L, O (A, B, C, D) | 5-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]-~{N}-[2-(3,4-dihydro-2~{H}-pyrano[2,3-c]pyridin-6-ylmethylamino)ethyl]pentanamide | non-polymer | 433.6 | 4 | Chemie (WZQ) PubChem (168477836) | |||
| 3 | F, G, J, K (A, B) | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) PubChem (27854) | |||
| 4 | H, M, N, P (A, C, D) | BROMIDE ION | non-polymer | 79.9 | 4 | Chemie (BR) PubChem (259) | |||
| 5 | Q (D) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) PubChem (312) | |||
| 6 | R, S, T, U (A, B, C, D) | water | water | 18.0 | 94 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 15 - 159 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ala 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Met 4 | - | expression tag |
| Thr 5 | - | expression tag |
| Gly 6 | - | expression tag |
| Gly 7 | - | expression tag |
| Gln 8 | - | expression tag |
| Gln 9 | - | expression tag |
| Met 10 | - | expression tag |
| Gly 11 | - | expression tag |
| Arg 12 | - | expression tag |
| Asp 13 | - | expression tag |
| Gln 14 | - | expression tag |
| Tyr 112 | Ser 136 | engineered mutation |
| Met 121 | Lys 145 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 66588.6 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 2309.1 | |
| All* | Total formula weight | 68897.7 |
