8P50

Photorhabdus luminescens Makes caterpillars floppy (Mcf) toxin with the C-terminal deletion in complex with Arf3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Toxin protein	polymer	2934	325211.6	1	UniProt (Q8KT65) Pfam (PF12920)	Photorhabdus luminescens
2	B (B)	ADP-ribosylation factor 3	polymer	184	20935.9	1	UniProt (P61204) Pfam (PF00025)	Homo sapiens (human)
3	C (B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
4	D (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 2914 (UniProt: Q8KT65)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 1397	Cys 1397	engineered mutation

B: 18 - 181 (UniProt: P61204)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
Gly 10	-	expression tag
Leu 11	-	expression tag
Val 12	-	expression tag
Pro 13	-	expression tag
Arg 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Leu 71	Gln 71	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	346147.5
Non-Polymers*	Number of molecules	2
	Total formula weight	547.5
All*	Total formula weight	346694.9

*Water molecules are not included.