8P4F
Structural insights into human co-transcriptional capping - structure 6
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (P) | RNA (29-MER) | polymer | 29 | 9368.7 | 1 | synthetic construct | ||
| 2 | B (A) | DNA-directed RNA polymerase II subunit RPB1 | polymer | 1970 | 217450.1 | 1 | UniProt (P11414) | Sus scrofa (pig) | |
| 3 | C (B) | DNA-directed RNA polymerase subunit beta | polymer | 1174 | 134041.4 | 1 | UniProt (I3LGP4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) | Sus scrofa (pig) | |
| 4 | D (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 275 | 31439.1 | 1 | UniProt (I3LCH3) Pfam (PF01193) Pfam (PF01000) | Sus scrofa (pig) | |
| 5 | E (E) | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24644.3 | 1 | UniProt (I3LSI7) Pfam (PF03871) Pfam (PF01191) | Sus scrofa (pig) | RPB5 homolog |
| 6 | F (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14491.0 | 1 | UniProt (F1SKN8) Pfam (PF01192) UniProt (by SIFTS) (A0A4X1VEK9) | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit F,RPB6 homolog |
| 7 | G (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (I3LCB2) Pfam (PF03870) | Sus scrofa (pig) | |
| 8 | H (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14507.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) | Sus scrofa (pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 9 | I (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A4X1VYD0) | Sus scrofa (pig) | |
| 10 | J (K) | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 117 | 13310.3 | 1 | UniProt (F1RKE4) Pfam (PF13656) | Sus scrofa (pig) | |
| 11 | K (L) | RNA polymerase II subunit K | polymer | 58 | 7018.2 | 1 | UniProt (I3LN51) Pfam (PF03604) UniProt (by SIFTS) (A0A4X1TRS6) | Sus scrofa (pig) | |
| 12 | L (N) | DNA (38-MER) | polymer | 38 | 11762.6 | 1 | synthetic construct | ||
| 13 | M (T) | DNA (38-MER) | polymer | 38 | 11628.5 | 1 | synthetic construct | ||
| 14 | N (Y) | Transcription elongation factor SPT4 | polymer | 117 | 13210.2 | 1 | UniProt (P63272) Pfam (PF06093) | Homo sapiens (human) | hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit |
| 15 | O (Z) | Transcription elongation factor SPT5 | polymer | 1087 | 121145.5 | 1 | UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) Pfam (PF23037) Pfam (PF23288) Pfam (PF23287) | Homo sapiens (human) | hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein |
| 16 | P (G) | DNA-directed RNA polymerase II subunit RPB7 | polymer | 172 | 19314.3 | 1 | UniProt (A0A4X1VKG7) | Sus scrofa (pig) | |
| 17 | Q (D) | RNA polymerase II subunit D | polymer | 142 | 16331.3 | 1 | UniProt (A0A287ADR4) UniProt (by SIFTS) (A0A4X1VM56) | Sus scrofa (pig) | |
| 18 | R (O) | Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 | polymer | 835 | 95454.8 | 1 | UniProt (Q8N1G2) Pfam (PF01585) Pfam (PF01728) | Homo sapiens (human) | Cap methyltransferase 1,Cap1 2'O-ribose methyltransferase 1,MTr1,hMTr1,FtsJ methyltransferase domain-containing protein 2,Interferon-stimulated gene 95 kDa protein,ISG95 |
| 19 | S (P) | 7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE | non-polymer | 539.2 | 1 | Chemie (MGT) | |||
| 20 | AA, BA, T, U, W... (L, Y, A, B, I...) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 21 | V (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 22 | CA (O) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) |
Sequence modifications
F: 1 - 127 (UniProt: F1SKN8)
I: 1 - 125 (UniProt: P60899)
| PDB | External Database | Details |
|---|---|---|
| Thr 126 | Ser 126 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ile 11 | Phe 11 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 779934.9 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1485.2 | |
| All* | Total formula weight | 781420.1 |
