8P3M

The structure of thiocyanate dehydrogenase mutant form with Lys 281 replaced by Ala from Thioalkalivibrio paradoxus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, D, G, J, M...)	Twin-arginine translocation signal domain-containing protein	polymer	494	54944.6	16	UniProt (W0DP94)	Thioalkalivibrio paradoxus ARh 1	Thiocyanate dehydrogenase
2	AB, CA, DB, FA, GB... (5, J, 8, M, x...)	SULFATE ION	non-polymer		96.1	17	Chemie (SO4)
3	R, W (A, D)	BORIC ACID	non-polymer		61.8	2	Chemie (BO3)
4	AA, BA, BB, CB, DA... (G, 5, J, 8, M...)	COPPER (II) ION	non-polymer		63.5	32	Chemie (CU)
5	MA, TA (P, V)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
6	AC, BC, CC, DC, EC... (2, 5, 8, x, e...)	water	water		18.0	2420	Chemie (HOH)

Sequence modifications

A, D, G, J, M, P, S, V, Y, 2, 5, 8, x, e, h, k: 82 - 548 (UniProt: W0DP94)

PDB	External Database	Details
Met 55	-	initiating methionine
Ser 56	-	expression tag
Tyr 57	-	expression tag
Tyr 58	-	expression tag
His 59	-	expression tag
His 60	-	expression tag
His 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
Asp 65	-	expression tag
Tyr 66	-	expression tag
Asp 67	-	expression tag
Ile 68	-	expression tag
Pro 69	-	expression tag
Thr 70	-	expression tag
Thr 71	-	expression tag
Glu 72	-	expression tag
Asn 73	-	expression tag
Leu 74	-	expression tag
Tyr 75	-	expression tag
Phe 76	-	expression tag
Gln 77	-	expression tag
Gly 78	-	expression tag
Ala 79	-	expression tag
Met 80	-	expression tag
Gly 81	-	expression tag
Ala 281	Lys 281	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	879113.5
Non-Polymers*	Number of molecules	53
	Total formula weight	3836.2
All*	Total formula weight	882949.7

*Water molecules are not included.