8P3I

Fusion hSlp2-a_Rab27A bound to covalent hit fragment IMP-1721

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Synaptotagmin-like protein 2,Ras-related protein Rab-27A	polymer	230	25914.2	2	UniProt (Q9HCH5) UniProt (P51159) Pfam (PF02318) Pfam (PF00071)	Homo sapiens (human)	Breast cancer-associated antigen SGA-72M,Exophilin-4,Rab-27,GTP-binding protein Ram
2	C, L (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	2	Chemie (GNP)
3	D, E, F, G, H... (A, B)	GLYCEROL	non-polymer		92.1	12	Chemie (GOL)
4	J, S (A, B)	~{N}-(3-methoxy-4-pyrrolidin-1-ylsulfonyl-phenyl)propanamide	non-polymer		312.4	2	Chemie (WTK)
5	K, T (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	U, V (A, B)	water	water		18.0	57	Chemie (HOH)

Sequence modifications

A, B: -35 - -9 (UniProt: Q9HCH5)

PDB	External Database	Details
Gly -38	-	expression tag
His -37	-	expression tag
Met -36	-	expression tag
Gly -9	-	linker
Ser -9	-	linker
Gly -9	-	linker
Ser -9	-	linker
Gly -9	-	linker
Ser -9	-	linker
Gly -9	-	linker

A, B: -9 - 179 (UniProt: P51159)

PDB	External Database	Details
Leu 65	Gln 78	engineered mutation
Ser 175	Cys 188	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51828.4
Non-Polymers*	Number of molecules	18
	Total formula weight	2822.9
All*	Total formula weight	54651.3

*Water molecules are not included.