8P3G

Fusion hSlp2-a_Rab27A bound to covalent hit fragment IMP-1704

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Synaptotagmin-like protein 2,Ras-related protein Rab-27A	polymer	230	25914.2	2	UniProt (Q9HCH5) UniProt (P51159) Pfam (PF02318) Pfam (PF00071)	Homo sapiens (human)	Breast cancer-associated antigen SGA-72M,Exophilin-4,Rab-27,GTP-binding protein Ram
2	C, E, F, I, K... (A, B)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
3	D, J (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	2	Chemie (GNP)
4	G, N (A, B)	~{N}-(4-pyrrolidin-1-ylsulfonylphenyl)propanamide	non-polymer		282.4	2	Chemie (WTU)
5	H, O (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	P, Q (A, B)	water	water		18.0	308	Chemie (HOH)

Sequence modifications

A, B: -35 - -11 (UniProt: Q9HCH5)

PDB	External Database	Details
Gly -38	-	expression tag
His -37	-	expression tag
Met -36	-	expression tag
Gly -11	-	linker
Ser -11	-	linker
Gly -11	-	linker
Ser -11	-	linker
Gly -11	-	linker
Ser -11	-	linker
Gly -11	-	linker

A, B: -11 - 176 (UniProt: P51159)

PDB	External Database	Details
Leu 62	Gln 78	engineered mutation
Ser 172	Cys 188	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51828.4
Non-Polymers*	Number of molecules	13
	Total formula weight	2302.4
All*	Total formula weight	54130.8

*Water molecules are not included.