8P15
Cryo-EM structure of Rhodopsin-Gi bound with antibody fragments scFv16 and Fab79, conformation 2
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Rhodopsin | polymer | 348 | 39040.5 | 1 | UniProt (P02699) Pfam (PF10413) Pfam (PF00001) | Bos taurus (cattle) | |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 376 | 43182.1 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37416.9 | 1 | UniProt (P62871) Pfam (PF00400) | Bos taurus (cattle) | Transducin beta chain 1 |
| 4 | D (G) | Guanine nucleotide-binding protein G(T) subunit gamma-T1 | polymer | 74 | 8556.9 | 1 | UniProt (P02698) Pfam (PF00631) | Bos taurus (cattle) | Transducin gamma chain |
| 5 | E (S) | single-chain Fv scFv16 | polymer | 259 | 27784.9 | 1 | Mus musculus | ||
| 6 | F (H) | Immunoglobin G heavy chain FAB fragment | polymer | 262 | 27972.0 | 1 | Mus musculus | ||
| 7 | G (L) | Immunoglobin G light chain | polymer | 239 | 26372.6 | 1 | Mus musculus |
Sequence modifications
R: 1 - 348 (UniProt: P02699)
A: 1 - 354 (UniProt: P63096)
| PDB | External Database | Details |
|---|---|---|
| Cys 2 | Asn 2 | engineered mutation |
| Tyr 257 | Met 257 | engineered mutation |
| Cys 282 | Asp 282 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -21 | - | initiating methionine |
| Lys -20 | - | expression tag |
| Lys -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 210325.9 | |
| All* | Total formula weight | 210325.9 |
