8P0T

CryoEM structure of 20S Trichomonas vaginalis proteasome in complex with proteasome inhibitor CP-17

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, O (A, O)	Family T1, proteasome alpha subunit, threonine peptidase	polymer	241	26511.2	2	UniProt (A2F568) Pfam (PF10584) Pfam (PF00227)	Trichomonas vaginalis G3
2	B, P (B, P)	Family T1, proteasome alpha subunit, threonine peptidase	polymer	232	25445.0	2	UniProt (A2FJV7) Pfam (PF00227)	Trichomonas vaginalis G3
3	C, Q (C, Q)	Proteasome subunit alpha type	polymer	251	27989.7	2	UniProt (A2FT79) Pfam (PF10584) Pfam (PF00227)	Trichomonas vaginalis G3
4	D, R (D, R)	Proteasome subunit alpha type	polymer	235	26306.9	2	UniProt (A2DTN3) Pfam (PF10584) Pfam (PF00227)	Trichomonas vaginalis G3
5	E, S (E, S)	Proteasome subunit alpha type	polymer	251	27331.0	2	UniProt (A2FCM7) Pfam (PF10584) Pfam (PF00227)	Trichomonas vaginalis G3
6	F, T (F, T)	Family T1, proteasome alpha subunit, threonine peptidase	polymer	233	25682.3	2	UniProt (A2E1I9) Pfam (PF10584) Pfam (PF00227)	Trichomonas vaginalis G3
7	G, U (G, U)	Family T1, proteasome alpha subunit, threonine peptidase	polymer	240	27067.4	2	UniProt (A2D8G5) Pfam (PF10584) Pfam (PF00227)	Trichomonas vaginalis G3
8	H, V (H, V)	Proteasome subunit beta	polymer	204	21930.8	2	UniProt (A2E7Z2) Pfam (PF00227)	Trichomonas vaginalis G3
9	I, W (I, W)	proteasome endopeptidase complex	polymer	243	26768.3	2	UniProt (A2F2T6) Pfam (PF00227)	Trichomonas vaginalis G3
10	J, X (J, X)	Proteasome subunit beta	polymer	206	23028.6	2	UniProt (A2F3H9) Pfam (PF00227)	Trichomonas vaginalis G3
11	K, Y (K, Y)	Proteasome subunit beta	polymer	191	21421.6	2	UniProt (A2F8W4) Pfam (PF00227)	Trichomonas vaginalis G3
12	L, Z (L, Z)	Proteasome subunit beta	polymer	202	22471.5	2	UniProt (A2DD57) Pfam (PF00227)	Trichomonas vaginalis G3
13	AA, M (M, a)	Proteasome subunit beta	polymer	224	25001.0	2	UniProt (A2F716) Pfam (PF00227)	Trichomonas vaginalis G3
14	BA, N (N, b)	Family T1, proteasome beta subunit, threonine peptidase	polymer	244	26486.6	2	UniProt (A2F3X4) Pfam (PF00227)	Trichomonas vaginalis G3
15	CA, DA, EA, FA (I, L, W, Z)	N-[(2S)-1-[[(2S)-1-[[(1S)-1-[(2S,3R,5S,6R)-3-(hydroxymethyl)-5-methanoyl-2,3,6-trimethyl-morpholin-2-yl]-2-phenyl-ethyl]amino]-3-(1H-indol-3-yl)-1-oxidanylidene-propan-2-yl]amino]-3-(1H-indol-3-yl)-1-oxidanylidene-propan-2-yl]hexanamide	non-polymer		777.0	4	Chemie (X5C)

Sequence modifications

N, b: 1 - 214 (UniProt: A2F3X4)

PDB	External Database	Details
Ser 215	-	expression tag
Ala 216	-	expression tag
Trp 217	-	expression tag
Ser 218	-	expression tag
His 219	-	expression tag
Pro 220	-	expression tag
Gln 221	-	expression tag
Phe 222	-	expression tag
Glu 223	-	expression tag
Lys 224	-	expression tag
Gly 225	-	expression tag
Gly 226	-	expression tag
Gly 227	-	expression tag
Ser 228	-	expression tag
Gly 229	-	expression tag
Gly 230	-	expression tag
Gly 231	-	expression tag
Ser 232	-	expression tag
Gly 233	-	expression tag
Gly 234	-	expression tag
Ser 235	-	expression tag
Ala 236	-	expression tag
Trp 237	-	expression tag
Ser 238	-	expression tag
His 239	-	expression tag
Pro 240	-	expression tag
Gln 241	-	expression tag
Phe 242	-	expression tag
Glu 243	-	expression tag
Lys 244	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	28
	Total formula weight	706883.9
Non-Polymers*	Number of molecules	4
	Total formula weight	3107.9
All*	Total formula weight	709991.8

*Water molecules are not included.