8P0D

Human 14-3-3 sigma in complex with human MDM2 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	14-3-3 protein sigma	polymer	259	29549.1	1	UniProt (P31947) Pfam (PF00244)	Homo sapiens (human)	Epithelial cell marker protein 1,Stratifin
2	B (B)	E3 ubiquitin-protein ligase Mdm2	polymer	31	3839.9	1	UniProt (Q00987)	Homo sapiens	Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
3	C (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	D (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	E (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	F, G (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	H, I (A, B)	water	water		18.0	367	Chemie (HOH)

Sequence modifications

A: 1 - 231 (UniProt: P31947)

PDB	External Database	Details
Met -27	-	initiating methionine
Ser -26	-	expression tag
Tyr -25	-	expression tag
Tyr -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Asp -17	-	expression tag
Tyr -16	-	expression tag
Asp -15	-	expression tag
Ile -14	-	expression tag
Pro -13	-	expression tag
Thr -12	-	expression tag
Thr -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	33389.0
Non-Polymers*	Number of molecules	5
	Total formula weight	282.3
All*	Total formula weight	33671.3

*Water molecules are not included.