8OY3

Time-resolved SFX structure of the class II photolyase complexed with a thymine dimer (3 picosecond pump-probe delay)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Deoxyribodipyrimidine photo-lyase	polymer	498	57039.7	2	UniProt (Q8PYK9) Pfam (PF00875) In PDB	Methanosarcina mazei Go1	DNA photolyase
2	C, E	CPD-COMPRISING OLIGONUCLEOTIDE	polymer	13	4256.8	2		synthetic construct
3	D, F	COUNTERSTRAND-OLIGONUCLEOTIDE	polymer	14	4305.8	2		synthetic construct
4	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	A, B	DIHYDROFLAVINE-ADENINE DINUCLEOTIDE	non-polymer		787.6	2	Chemie (FDA)
6		water	water		18.0	239	Chemie (HOH)

Sequence modifications

A, B: 1 - 464 (UniProt: Q8PYK9)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 465	-	expression tag
Lys 466	-	expression tag
Leu 467	-	expression tag
Ala 468	-	expression tag
Ala 469	-	expression tag
Ala 470	-	expression tag
Leu 471	-	expression tag
Glu 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	131204.6
Non-Polymers*	Number of molecules	3
	Total formula weight	1671.2
All*	Total formula weight	132875.8

*Water molecules are not included.