8OWU

The crystal structure of the polymorphic toxin PT7(Bc) D37A mutant and its cognate immunity PIM7(Bc) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, C)	Transposase	polymer	95	11149.5	2	UniProt (A0A2A8X9H8) UniProt (by SIFTS) (A0AAT8XUD1)	Bacillus cereus BAG3X2-1	PT7,PT7Bc
2	C, D (D, E)	PIM7	polymer	117	14027.3	2	UniProt (A0A2C1PRY7) UniProt (by SIFTS) (A0AAT8XUD2)	Bacillus cereus BAG3X2-1	PIM7Bc
3	E (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

B, C: 1 - 78 (UniProt: A0A2A8X9H8)

PDB	External Database	Details
Met -16	-	initiating methionine
Gly -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ala 37	Asp 37	engineered mutation

D, E: 1 - 117 (UniProt: A0A2C1PRY7)

PDB	External Database	Details
Ile 59	Met 59	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	50353.7
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	50378.0

*Water molecules are not included.