8OWS

The crystal structure of the polymorphic toxin PT1(Em) H44A mutant and its cognate immunity PIM1(Em) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	PIM1	polymer	122	14297.8	1	UniProt (A0A370V6P8)	Escherichia marmotae	PIM1Em
2	B (C)	Type IV secretion protein Rhs	polymer	135	15308.1	1	UniProt (A0A370V6R2)	Escherichia marmotae	PT1, PT1Em
3	C, D (A, C)	water	water		18.0	205	Chemie (HOH)

Sequence modifications

C: 1 - 119 (UniProt: A0A370V6R2)

PDB	External Database	Details
Gly -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ala 44	His 44	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	29605.9
All*	Total formula weight	29605.9

*Water molecules are not included.