8OT0

SARS-CoV-2 nsp10-16 methyltransferase in complex with MTA and glycine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	2'-O-methyltransferase nsp16	polymer	304	34140.1	1	UniProt (P0DTD1) Pfam (PF06460)	Severe acute respiratory syndrome coronavirus 2	Non-structural protein 16,nsp16
2	B (B)	Non-structural protein 10	polymer	140	14860.0	1	UniProt (P0DTD1) Pfam (PF09401)	Severe acute respiratory syndrome coronavirus 2	nsp10,Growth factor-like peptide,GFL
3	AA, C, D, EA, F... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	25	Chemie (EDO)
4	E (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES) PubChem (4478249)
5	S (A)	5'-DEOXY-5'-METHYLTHIOADENOSINE	non-polymer		297.3	1	Chemie (MTA) PubChem (149) PubChem (25322898) PubChem (40467850) PubChem (44264468) PubChem (46874793) PubChem (51397471) PubChem (57104771) PubChem (58108938) PubChem (58682801) PubChem (439176) PubChem (59099797) PubChem (51397472) PubChem (688097) PubChem (99302) PubChem (453683) PubChem (91614589) PubChem (98100246) PubChem (6541356) PubChem (124300729) PubChem (124300795) PubChem (13101165) PubChem (13101166) PubChem (134694842) PubChem (138107656) PubChem (164077596)
6	BA (A)	GLYCINE	non-polymer		75.1	1	Chemie (GLY) PubChem (750)
7	CA, DA (B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
8	GA, HA (A, B)	water	water		18.0	190	Chemie (HOH)

Sequence modifications

A: 6799 - 7096 (UniProt: P0DTD1)

PDB	External Database	Details
Glu 7097	-	expression tag
Asn 7098	-	expression tag
Cys 7099	-	expression tag
Tyr 7100	-	expression tag
Phe 7101	-	expression tag
Gln 7102	-	expression tag

B: 4254 - 4392 (UniProt: P0DTD1)

PDB	External Database	Details
Gly 4253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49000.1
Non-Polymers*	Number of molecules	30
	Total formula weight	2250.2
All*	Total formula weight	51250.2

*Water molecules are not included.