8OQY

Structure of apo form of human gamma-secretase PSEN1 APH-1B isoform reconstituted into lipid nanodisc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Nicastrin	polymer	717	79371.6	1	UniProt (Q92542) Pfam (PF18266) Pfam (PF05450)	Homo sapiens (human)
2	B (B)	Presenilin-1 CTF12	polymer	467	52713.5	1	UniProt (P49768) Pfam (PF01080)	Homo sapiens (human)	PS1-CTF12
3	C (C)	Gamma-secretase subunit APH-1B	polymer	257	28480.8	1	UniProt (Q8WW43) Pfam (PF06105)	Homo sapiens (human)	APH-1b,Aph-1beta,Presenilin-stabilization factor-like
4	D (D)	Gamma-secretase subunit PEN-2	polymer	101	12038.0	1	UniProt (Q9NZ42) Pfam (PF10251)	Homo sapiens (human)	Presenilin enhancer protein 2
5	E, G, H (E, G, H)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
6	F (F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		789.7	1	In PDB GlyTouCan (G95663PY)
7	I, J, K, L, M... (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)
8	P, Q, R (A, B, C)	1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		790.1	3	Chemie (PC1)

Sequence modifications

A: 1 - 709 (UniProt: Q92542)

PDB	External Database	Details
Gly 710	-	expression tag
Thr 711	-	expression tag
Leu 712	-	expression tag
Glu 713	-	expression tag
Val 714	-	expression tag
Leu 715	-	expression tag
Phe 716	-	expression tag
Gln 717	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	172604.0
Branched	Number of molecules	4
	Total formula weight	2062.9
Non-Polymers*	Number of molecules	10
	Total formula weight	3918.9
All*	Total formula weight	178585.8

*Water molecules are not included.