8OO1
Wide inward-open liganded UraA in complex with a conformation-selective synthetic nanobody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C | Uracil permease | polymer | 437 | 46017.9 | 2 | UniProt (P0AGM8) Pfam (PF00860) UniProt (by SIFTS) (P0AGM7) In PDB | Escherichia coli O157:H7 | Uracil transporter,Uracil/H(+) symporter UraA |
| 2 | B, D | Sy45 | polymer | 154 | 16668.3 | 2 | synthetic construct | ||
| 3 | A, D | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 2 | Chemie (1PE) | |||
| 4 | A, C | nonyl beta-D-glucopyranoside | non-polymer | 306.4 | 5 | Chemie (BNG) | |||
| 5 | A, C | URACIL | non-polymer | 112.1 | 2 | Chemie (URA) | |||
| 6 | B, D | DECYL-BETA-D-MALTOPYRANOSIDE | non-polymer | 482.6 | 2 | Chemie (DMU) |
Sequence modifications
A, C: 2 - 429 (UniProt: P0AGM8)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Ser 1 | - | expression tag |
| Pro 320 | Gly 320 | engineered mutation |
| Ala 430 | - | expression tag |
| Leu 431 | - | expression tag |
| Glu 432 | - | expression tag |
| Val 433 | - | expression tag |
| Leu 434 | - | expression tag |
| Phe 435 | - | expression tag |
| Gln 436 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 125372.4 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 3197.8 | |
| All* | Total formula weight | 128570.2 |
