8OKI

Cryo-EM structure of Pyrococcus furiosus transcription elongation complex bound to Spt4/5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed RNA polymerase subunit Rpo1N	polymer	910	103612.3	1	UniProt (A0A5C0XPL7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (Q8U0M4) In PDB	Pyrococcus furiosus DSM 3638	DNA-directed RNA polymerase subunit A'
2	B	DNA-directed RNA polymerase subunit beta	polymer	1117	127188.9	1	UniProt (Q8U0M3) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) In PDB	Pyrococcus furiosus DSM 3638
3	C	DNA-directed RNA polymerase subunit Rpo1C	polymer	397	44468.2	1	UniProt (Q8U0M5) Pfam (PF04998) In PDB	Pyrococcus furiosus DSM 3638	DNA-directed RNA polymerase subunit A''
4	D	DNA-directed RNA polymerase subunit Rpo3	polymer	275	31688.2	1	UniProt (Q8U0E4) Pfam (PF01193) Pfam (PF01000) In PDB	Pyrococcus furiosus DSM 3638	DNA-directed RNA polymerase subunit D
5	G	Transcription elongation factor Spt5	polymer	152	16816.6	1	UniProt (Q8TZK1) Pfam (PF03439) Pfam (PF00467) In PDB	Pyrococcus furiosus DSM 3638
6	H	DNA-directed RNA polymerase subunit Rpo5	polymer	82	9260.8	1	UniProt (Q8U0M2) Pfam (PF01191) In PDB	Pyrococcus furiosus DSM 3638	DNA-directed RNA polymerase subunit H
7	I	Transcription elongation factor Spt4	polymer	61	6965.0	1	UniProt (Q8U440) Pfam (PF06093) In PDB	Pyrococcus furiosus DSM 3638
8	K	DNA-directed RNA polymerase subunit Rpo6	polymer	57	6243.5	1	UniProt (Q8U0E8) Pfam (PF01192) In PDB	Pyrococcus furiosus DSM 3638	DNA-directed RNA polymerase subunit K
9	L	DNA-directed RNA polymerase subunit Rpo11	polymer	95	11132.8	1	UniProt (Q8U4N1) Pfam (PF13656) In PDB	Pyrococcus furiosus DSM 3638	DNA-directed RNA polymerase subunit L
10	N	DNA-directed RNA polymerase subunit Rpo10	polymer	65	7800.2	1	UniProt (P60292) Pfam (PF01194) In PDB	Pyrococcus furiosus DSM 3638	DNA-directed RNA polymerase subunit N
11	P	DNA-directed RNA polymerase subunit Rpo12	polymer	49	5770.1	1	UniProt (Q8TZI3) Pfam (PF03604) In PDB	Pyrococcus furiosus DSM 3638	DNA-directed RNA polymerase subunit P
12	X	DNA Template Strand	polymer	26	8043.2	1		Pyrococcus furiosus DSM 3638
13	Y	DNA Non-Template Strand	polymer	26	7932.1	1		Pyrococcus furiosus DSM 3638
14	Z	RNA	polymer	9	2909.8	1		Pyrococcus furiosus DSM 3638
15	E	DNA-directed RNA polymerase subunit Rpo7	polymer	189	21731.5	1	UniProt (Q8U439) Pfam (PF03876) Pfam (PF00575) In PDB	Pyrococcus furiosus DSM 3638
16	F	DNA-directed RNA polymerase subunit Rpo4	polymer	120	14116.3	1	UniProt (Q8U216) Pfam (PF03874) In PDB	Pyrococcus furiosus DSM 3638	DNA-directed RNA polymerase subunit F
17	A, B, I, N, P	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
18	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

D: 1 - 261 (UniProt: Q8U0E4)

PDB	External Database	Details
Trp 262	-	expression tag
Ser 263	-	expression tag
His 264	-	expression tag
Pro 265	-	expression tag
Gln 266	-	expression tag
Phe 267	-	expression tag
Glu 268	-	expression tag
Lys 269	-	expression tag
His 270	-	expression tag
His 271	-	expression tag
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	425679.3
Non-Polymers*	Number of molecules	7
	Total formula weight	416.8
All*	Total formula weight	426096.1

*Water molecules are not included.