8OE2
Structure of hyperstable haloalkane dehalogenase variant DhaA223
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... | Haloalkane dehalogenase | polymer | 299 | 34642.5 | 6 | UniProt (P0A3G4) Pfam (PF00561) In PDB | synthetic construct | |
| 2 | E, A, F, B, C... | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
| 3 | E, A, B, C, D | SULFATE ION | non-polymer | 96.1 | 7 | Chemie (SO4) | |||
| 4 | E, A, D | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 3 | Chemie (PG4) | |||
| 5 | D, E, F, A, B... | GLYCEROL | non-polymer | 92.1 | 12 | Chemie (GOL) | |||
| 6 | A | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 7 | water | water | 18.0 | 1853 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 1 - 293 (UniProt: P0A3G4)
| PDB | External Database | Details |
|---|---|---|
| Ser 20 | Glu 20 | conflict |
| Arg 80 | Phe 80 | conflict |
| Phe 128 | Cys 128 | conflict |
| Leu 148 | Thr 148 | conflict |
| Pro 155 | Ala 155 | conflict |
| Tyr 165 | Gln 165 | conflict |
| Ile 171 | Gly 171 | conflict |
| Ile 172 | Ala 172 | conflict |
| Phe 176 | Cys 176 | conflict |
| Glu 184 | Val 184 | conflict |
| Glu 197 | Val 197 | conflict |
| Trp 198 | Asp 198 | conflict |
| Asn 212 | Ala 212 | conflict |
| Asp 217 | Asn 217 | conflict |
| Trp 219 | Val 219 | conflict |
| Leu 262 | Cys 262 | conflict |
| Phe 266 | Asp 266 | conflict |
| His 294 | - | expression tag |
| His 295 | - | expression tag |
| His 296 | - | expression tag |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
| His 299 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 207855.0 | |
| Non-Polymers* | Number of molecules | 29 |
| Total formula weight | 2695.1 | |
| All* | Total formula weight | 210550.2 |
