8KIE

Structure of YchF with 50S ribosomal subunit (local map)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	y	Ribosome-binding ATPase YchF	polymer	379	41651.3	1	UniProt (P0ABU2) Pfam (PF01926) Pfam (PF06071) In PDB	Escherichia coli
2	a	23S rRNA (partial)	polymer	2904	941663.4	1		Escherichia coli
3	j	50S ribosomal protein L14	polymer	123	13565.1	1	UniProt (P0ADY3) Pfam (PF00238) In PDB	Escherichia coli	Large ribosomal subunit protein uL14
4	o	50S ribosomal protein L19	polymer	115	13159.3	1	UniProt (P0A7K6) Pfam (PF01245) In PDB	Escherichia coli	Large ribosomal subunit protein bL19
5	a	MAGNESIUM ION	non-polymer		24.3	30	Chemie (MG)

Sequence modifications

y: 1 - 363 (UniProt: P0ABU2)

PDB	External Database	Details
Met -15	-	initiating methionine
Gly -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Ala 114	His 114	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	1010039.1
Non-Polymers*	Number of molecules	30
	Total formula weight	729.1
All*	Total formula weight	1010768.2

*Water molecules are not included.