8KGK

Cryo-EM structure of the GPR61-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	G-protein coupled receptor 61	polymer	390	42527.6	1	UniProt (Q9BZJ8) Pfam (PF00001) In PDB	Homo sapiens (human)	Biogenic amine receptor-like G-protein coupled receptor
2	B	Guanine nucleotide-binding protein G(olf) subunit alpha,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	249	29008.8	1	UniProt (P38405) UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein,olfactory type,Adenylate cyclase-stimulating G alpha protein
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	357	39286.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
4	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	E	Nanobody 35	polymer	160	17352.5	1		Lama glama

Sequence modifications

A: 1 - 365 (UniProt: Q9BZJ8)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
Ala 0	-	expression tag
Leu 366	-	expression tag
Glu 367	-	expression tag
Val 368	-	expression tag
Leu 369	-	expression tag
Phe 370	-	expression tag
Gln 371	-	expression tag
Gly 372	-	expression tag
Pro 373	-	expression tag
Leu 374	-	expression tag
Glu 375	-	expression tag
Val 376	-	expression tag
Leu 377	-	expression tag
Phe 378	-	expression tag
Gln 379	-	expression tag
Gly 380	-	expression tag
Pro 381	-	expression tag

B: 5 - 195 (UniProt: P38405)

PDB	External Database	Details
Arg 13	Gly 15	engineered mutation
Asn 14	Val 16	engineered mutation
Glu 15	Asp 17	engineered mutation
Ala 18	Glu 20	engineered mutation
Gln 19	Arg 21	engineered mutation
Asp 33	Glu 35	engineered mutation
Lys 34	Arg 36	engineered mutation
Gln 35	Leu 37	engineered mutation
Val 36	Ala 38	engineered mutation
Arg 38	Lys 40	engineered mutation
Asp 49	Gly 51	engineered mutation
Asn 50	Glu 52	engineered mutation
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker

B: 204 - 384 (UniProt: P63092)

PDB	External Database	Details
Asp 249	Ala 249	engineered mutation
Asp 252	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 362	Ile 372	engineered mutation
Ile 365	Val 375	engineered mutation

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

D: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	136020.9
All*	Total formula weight	136020.9

*Water molecules are not included.