8KGK
Cryo-EM structure of the GPR61-Gs complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | G-protein coupled receptor 61 | polymer | 390 | 42527.6 | 1 | UniProt (Q9BZJ8) Pfam (PF00001) In PDB | Homo sapiens (human) | Biogenic amine receptor-like G-protein coupled receptor |
2 | B | Guanine nucleotide-binding protein G(olf) subunit alpha,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 249 | 29008.8 | 1 | UniProt (P38405) UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein,olfactory type,Adenylate cyclase-stimulating G alpha protein |
3 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 357 | 39286.9 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
4 | D | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7845.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
5 | E | Nanobody 35 | polymer | 160 | 17352.5 | 1 | Lama glama |
Sequence modifications
A: 1 - 365 (UniProt: Q9BZJ8)
B: 5 - 195 (UniProt: P38405)
B: 204 - 384 (UniProt: P63092)
C: 2 - 340 (UniProt: P62873)
D: 1 - 71 (UniProt: P59768)
PDB | External Database | Details |
---|---|---|
Asp -8 | - | expression tag |
Tyr -7 | - | expression tag |
Lys -6 | - | expression tag |
Asp -5 | - | expression tag |
Asp -4 | - | expression tag |
Asp -3 | - | expression tag |
Asp -2 | - | expression tag |
Lys -1 | - | expression tag |
Ala 0 | - | expression tag |
Leu 366 | - | expression tag |
Glu 367 | - | expression tag |
Val 368 | - | expression tag |
Leu 369 | - | expression tag |
Phe 370 | - | expression tag |
Gln 371 | - | expression tag |
Gly 372 | - | expression tag |
Pro 373 | - | expression tag |
Leu 374 | - | expression tag |
Glu 375 | - | expression tag |
Val 376 | - | expression tag |
Leu 377 | - | expression tag |
Phe 378 | - | expression tag |
Gln 379 | - | expression tag |
Gly 380 | - | expression tag |
Pro 381 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Arg 13 | Gly 15 | engineered mutation |
Asn 14 | Val 16 | engineered mutation |
Glu 15 | Asp 17 | engineered mutation |
Ala 18 | Glu 20 | engineered mutation |
Gln 19 | Arg 21 | engineered mutation |
Asp 33 | Glu 35 | engineered mutation |
Lys 34 | Arg 36 | engineered mutation |
Gln 35 | Leu 37 | engineered mutation |
Val 36 | Ala 38 | engineered mutation |
Arg 38 | Lys 40 | engineered mutation |
Asp 49 | Gly 51 | engineered mutation |
Asn 50 | Glu 52 | engineered mutation |
Gly 196 | - | linker |
Gly 197 | - | linker |
Ser 198 | - | linker |
Gly 199 | - | linker |
Gly 200 | - | linker |
Ser 201 | - | linker |
Gly 202 | - | linker |
Gly 203 | - | linker |
PDB | External Database | Details |
---|---|---|
Asp 249 | Ala 249 | engineered mutation |
Asp 252 | Ser 252 | engineered mutation |
- | Asn 254 | deletion |
- | Met 255 | deletion |
- | Val 256 | deletion |
- | Ile 257 | deletion |
- | Arg 258 | deletion |
- | Glu 259 | deletion |
- | Asp 260 | deletion |
- | Asn 261 | deletion |
- | Gln 262 | deletion |
- | Thr 263 | deletion |
Ala 362 | Ile 372 | engineered mutation |
Ile 365 | Val 375 | engineered mutation |
PDB | External Database | Details |
---|---|---|
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Leu -10 | - | expression tag |
Glu -9 | - | expression tag |
Val -8 | - | expression tag |
Leu -7 | - | expression tag |
Phe -6 | - | expression tag |
Gln -5 | - | expression tag |
Gly -4 | - | expression tag |
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ser 68 | Cys 68 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 136020.9 | |
All* | Total formula weight | 136020.9 |