8KGG
LPS-bound P2Y10 in complex with G13
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(i) subunit alpha-2,Guanine nucleotide-binding protein subunit alpha-13 | polymer | 230 | 26574.4 | 1 | UniProt (P04899) UniProt (Q14344) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein,G alpha-13,G-protein subunit alpha-13 |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
3 | E | scFv16 | polymer | 247 | 26277.3 | 1 | Homo sapiens | ||
4 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
5 | R | Putative P2Y purinoceptor 10 | polymer | 339 | 38812.1 | 1 | UniProt (O00398) Pfam (PF00001) In PDB | Homo sapiens (human) | |
6 | R | (2~{S})-2-$l^{4}-azanyl-3-[[(2~{R})-3-octadecanoyloxy-2-oxidanyl-propoxy]-oxidanyl-oxidanylidene-$l^{6}-phosphanyl]oxy-propanoic acid | non-polymer | 497.4 | 1 | Chemie (WJS) |
Sequence modifications
A: 1 - 57 (UniProt: P04899)
A: 66 - 230 (UniProt: Q14344)
B: 2 - 340 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Ser 3 | Cys 3 | engineered mutation |
Glu 18 | Met 18 | engineered mutation |
Cys 22 | Asn 22 | engineered mutation |
Ser 24 | Arg 24 | engineered mutation |
Arg 25 | Glu 25 | engineered mutation |
Glu 26 | Asp 26 | engineered mutation |
Lys 27 | Gly 27 | engineered mutation |
Thr 28 | Glu 28 | engineered mutation |
Tyr 29 | Lys 29 | engineered mutation |
Val 30 | Ala 30 | engineered mutation |
Lys 31 | Ala 31 | engineered mutation |
Leu 33 | Glu 33 | engineered mutation |
Ile 36 | Leu 36 | engineered mutation |
Asp 42 | Gly 42 | engineered mutation |
Asn 43 | Glu 43 | engineered mutation |
Phe 49 | Ile 49 | engineered mutation |
Leu 50 | Val 50 | engineered mutation |
Arg 54 | Lys 54 | engineered mutation |
Gly 58 | - | linker |
Gly 59 | - | linker |
Ser 60 | - | linker |
Gly 61 | - | linker |
Gly 62 | - | linker |
Ser 63 | - | linker |
Gly 64 | - | linker |
Gly 65 | - | linker |
PDB | External Database | Details |
---|---|---|
Asp 111 | Ser 248 | engineered mutation |
Asp 114 | Glu 251 | engineered mutation |
- | Asp 253 | deletion |
- | Gln 254 | deletion |
- | Val 255 | deletion |
- | Leu 256 | deletion |
- | Met 257 | deletion |
- | Glu 258 | deletion |
- | Asp 259 | deletion |
- | Arg 260 | deletion |
- | Leu 261 | deletion |
- | Thr 262 | deletion |
Asp 124 | Ile 271 | engineered mutation |
Ala 208 | Ile 355 | engineered mutation |
Ile 211 | Val 358 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 137440.4 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 497.4 | |
All* | Total formula weight | 137937.8 |