8KGG
LPS-bound P2Y10 in complex with G13
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-2,Guanine nucleotide-binding protein subunit alpha-13 | polymer | 230 | 26574.4 | 1 | UniProt (P04899) UniProt (Q14344) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein,G alpha-13,G-protein subunit alpha-13 |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (E) | scFv16 | polymer | 247 | 26277.3 | 1 | Homo sapiens | ||
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (R) | Putative P2Y purinoceptor 10 | polymer | 339 | 38812.1 | 1 | UniProt (O00398) Pfam (PF00001) | Homo sapiens (human) | |
| 6 | F (R) | (2~{S})-2-$l^{4}-azanyl-3-[[(2~{R})-3-octadecanoyloxy-2-oxidanyl-propoxy]-oxidanyl-oxidanylidene-$l^{6}-phosphanyl]oxy-propanoic acid | non-polymer | 497.4 | 1 | Chemie (WJS) |
Sequence modifications
A: 1 - 57 (UniProt: P04899)
A: 66 - 230 (UniProt: Q14344)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Ser 3 | Cys 3 | engineered mutation |
| Glu 18 | Met 18 | engineered mutation |
| Cys 22 | Asn 22 | engineered mutation |
| Ser 24 | Arg 24 | engineered mutation |
| Arg 25 | Glu 25 | engineered mutation |
| Glu 26 | Asp 26 | engineered mutation |
| Lys 27 | Gly 27 | engineered mutation |
| Thr 28 | Glu 28 | engineered mutation |
| Tyr 29 | Lys 29 | engineered mutation |
| Val 30 | Ala 30 | engineered mutation |
| Lys 31 | Ala 31 | engineered mutation |
| Leu 33 | Glu 33 | engineered mutation |
| Ile 36 | Leu 36 | engineered mutation |
| Asp 42 | Gly 42 | engineered mutation |
| Asn 43 | Glu 43 | engineered mutation |
| Phe 49 | Ile 49 | engineered mutation |
| Leu 50 | Val 50 | engineered mutation |
| Arg 54 | Lys 54 | engineered mutation |
| Gly 58 | - | linker |
| Gly 59 | - | linker |
| Ser 60 | - | linker |
| Gly 61 | - | linker |
| Gly 62 | - | linker |
| Ser 63 | - | linker |
| Gly 64 | - | linker |
| Gly 65 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Asp 111 | Ser 248 | engineered mutation |
| Asp 114 | Glu 251 | engineered mutation |
| - | Asp 253 | deletion |
| - | Gln 254 | deletion |
| - | Val 255 | deletion |
| - | Leu 256 | deletion |
| - | Met 257 | deletion |
| - | Glu 258 | deletion |
| - | Asp 259 | deletion |
| - | Arg 260 | deletion |
| - | Leu 261 | deletion |
| - | Thr 262 | deletion |
| Asp 124 | Ile 271 | engineered mutation |
| Ala 208 | Ile 355 | engineered mutation |
| Ile 211 | Val 358 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 137440.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 497.4 | |
| All* | Total formula weight | 137937.8 |
