8KE2
PylRS C-terminus domain mutant bound with L-3-trifluoromethylphenylalanine and AMPNP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pyrrolysine--tRNA ligase | polymer | 277 | 31999.6 | 1 | UniProt (A0A0F8JXW8) UniProt (by SIFTS) (Q8PWY1) | Methanosarcina mazei | Pyrrolysyl-tRNA synthetase,PylRS |
| 2 | B, C (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | D (A) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 4 | E (A) | (2S)-2-azanyl-3-[3-(trifluoromethyl)phenyl]propanoic acid | non-polymer | 233.2 | 1 | Chemie (FX9) | |||
| 5 | F (A) | water | water | 18.0 | 133 | Chemie (HOH) |
Sequence modifications
A: 185 - 454 (UniProt: A0A0F8JXW8)
| PDB | External Database | Details |
|---|---|---|
| Met 178 | - | expression tag |
| His 179 | - | expression tag |
| His 180 | - | expression tag |
| His 181 | - | expression tag |
| His 182 | - | expression tag |
| His 183 | - | expression tag |
| His 184 | - | expression tag |
| Gly 346 | Asn 346 | engineered mutation |
| Gln 348 | Cys 348 | engineered mutation |
| Gly 401 | Val 401 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31999.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 788.0 | |
| All* | Total formula weight | 32787.6 |
