8KD4

Rpd3S in complex with nucleosome with H3K36MLA modification and 187bp DNA, class1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D, F	Chromatin modification-related protein EAF3	polymer	401	45266.4	2	UniProt (Q12432) Pfam (PF11717) Pfam (PF22732) Pfam (PF05712) In PDB	Saccharomyces cerevisiae (baker's yeast)	ESA1-associated factor 3
2	O, S	Histone H3	polymer	135	15313.9	2	UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) In PDB	Xenopus laevis (African clawed frog)
3	P, T	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
4	Q, U	Histone H2A	polymer	129	13978.2	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) In PDB	Xenopus laevis (African clawed frog)
5	R, V	Histone H2B 1.1	polymer	122	13524.8	2	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
6	X	187bp DNA	polymer	187	58077.9	1		synthetic construct
7	Y	187bp DNA	polymer	187	57397.5	1		synthetic construct
8	A	Histone deacetylase RPD3	polymer	433	48962.0	1	UniProt (P32561) Pfam (PF00850) In PDB	Saccharomyces cerevisiae (baker's yeast)
9	B	Transcriptional regulatory protein SIN3	polymer	1371	157586.8	1	UniProt (P22579) Pfam (PF02671) Pfam (PF08295) Pfam (PF16879) In PDB	Saccharomyces cerevisiae (baker's yeast)
10	E, G	Transcriptional regulatory protein RCO1	polymer	733	84469.3	2	UniProt (Q04779) Pfam (PF00628) In PDB	Saccharomyces cerevisiae (baker's yeast)
11	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

O, S: 1 - 135 (UniProt: A0A310TTQ1)

PDB	External Database	Details
Ala 110	Cys 111	engineered mutation

R, V: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	engineered mutation

B: 215 - 1536 (UniProt: P22579)

PDB	External Database	Details
Met 166	-	initiating methionine
His 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag
Pro 175	-	expression tag
Gln 176	-	expression tag
Leu 177	-	expression tag
Ala 178	-	expression tag
Met 179	-	expression tag
Trp 180	-	expression tag
Ser 181	-	expression tag
His 182	-	expression tag
Pro 183	-	expression tag
Gln 184	-	expression tag
Phe 185	-	expression tag
Glu 186	-	expression tag
Lys 187	-	expression tag
Gly 188	-	expression tag
Gly 189	-	expression tag
Gly 190	-	expression tag
Ser 191	-	expression tag
Gly 192	-	expression tag
Gly 193	-	expression tag
Gly 194	-	expression tag
Ser 195	-	expression tag
Gly 196	-	expression tag
Gly 197	-	expression tag
Gly 198	-	expression tag
Ser 199	-	expression tag
Trp 200	-	expression tag
Ser 201	-	expression tag
His 202	-	expression tag
Pro 203	-	expression tag
Gln 204	-	expression tag
Phe 205	-	expression tag
Glu 206	-	expression tag
Lys 207	-	expression tag
Glu 208	-	expression tag
Asn 209	-	expression tag
Leu 210	-	expression tag
Tyr 211	-	expression tag
Phe 212	-	expression tag
Gln 213	-	expression tag
Ser 214	-	expression tag

E, G: 1 - 684 (UniProt: Q04779)

PDB	External Database	Details
Met 685	-	expression tag
His 686	-	expression tag
His 687	-	expression tag
His 688	-	expression tag
His 689	-	expression tag
His 690	-	expression tag
His 691	-	expression tag
His 692	-	expression tag
His 693	-	expression tag
Pro 694	-	expression tag
Gln 695	-	expression tag
Leu 696	-	expression tag
Ala 697	-	expression tag
Met 698	-	expression tag
Trp 699	-	expression tag
Ser 700	-	expression tag
His 701	-	expression tag
Pro 702	-	expression tag
Gln 703	-	expression tag
Phe 704	-	expression tag
Glu 705	-	expression tag
Lys 706	-	expression tag
Gly 707	-	expression tag
Gly 708	-	expression tag
Gly 709	-	expression tag
Ser 710	-	expression tag
Gly 711	-	expression tag
Gly 712	-	expression tag
Gly 713	-	expression tag
Ser 714	-	expression tag
Gly 715	-	expression tag
Gly 716	-	expression tag
Gly 717	-	expression tag
Ser 718	-	expression tag
Trp 719	-	expression tag
Ser 720	-	expression tag
His 721	-	expression tag
Pro 722	-	expression tag
Gln 723	-	expression tag
Phe 724	-	expression tag
Glu 725	-	expression tag
Lys 726	-	expression tag
Glu 727	-	expression tag
Asn 728	-	expression tag
Leu 729	-	expression tag
Tyr 730	-	expression tag
Phe 731	-	expression tag
Gln 732	-	expression tag
Ser 733	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	689656.0
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	689721.4

*Water molecules are not included.