8KCW

Cryo-EM structure of human SIDT1 bound to cholesterol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	SID1 transmembrane family member 1	polymer	850	96877.3	2	UniProt (Q9NXL6) Pfam (PF13965)	Homo sapiens (human)
2	C, D, G, H (G, H, M, N)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G15407YE)
3	E, F, I, J (I, J, O, P)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
4	K, N (A, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	L, M, O, P (A, C)	CHOLESTEROL	non-polymer		386.7	4	Chemie (CLR)

Sequence modifications

A, C: 1 - 827 (UniProt: Q9NXL6)

PDB	External Database	Details
Glu 828	-	expression tag
Asn 829	-	expression tag
Leu 830	-	expression tag
Tyr 831	-	expression tag
Phe 832	-	expression tag
Gln 833	-	expression tag
Gly 834	-	expression tag
Asp 835	-	expression tag
Tyr 836	-	expression tag
Lys 837	-	expression tag
Asp 838	-	expression tag
Asp 839	-	expression tag
Asp 840	-	expression tag
Asp 841	-	expression tag
Lys 842	-	expression tag
His 843	-	expression tag
His 844	-	expression tag
His 845	-	expression tag
His 846	-	expression tag
His 847	-	expression tag
His 848	-	expression tag
His 849	-	expression tag
His 850	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	193754.6
Branched	Number of molecules	8
	Total formula weight	4043.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1677.4
All*	Total formula weight	199475.8

*Water molecules are not included.