8KA0

Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin and a nicotinamide adenine dinucleotide (NAD+)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	RDTND-RID CBD	polymer	416	47092.2	4	UniProt (A0A2S3R7M0) In PDB	Vibrio vulnificus	MARTX
2	B, D, F, H	Calmodulin-2	polymer	151	16979.7	4	UniProt (P0DP24) Pfam (PF13499) In PDB	Homo sapiens (human)
3	A, C, E, G	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
4	B, D, F, H	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
5	C, D, F	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
6		water	water		18.0	616	Chemie (HOH)

Sequence modifications

A, C, E, G: 1959 - 2374 (UniProt: A0A2S3R7M0)

PDB	External Database	Details
Gln 2186	Glu 2186	engineered mutation

B, D, F, H: 1 - 149 (UniProt: P0DP24)

PDB	External Database	Details
Gly -1	-	cloning artifact
Ala 0	-	cloning artifact
Gln 124	Glu 124	conflict

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	256287.4
Non-Polymers*	Number of molecules	16
	Total formula weight	3342.7
All*	Total formula weight	259630.1

*Water molecules are not included.