8K9R
Cryo EM structure of the products-bound PGAP1(Bst1)-H443N from Chaetomium thermophilum
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | GPI inositol-deacylase,MCherry protein | polymer | 1447 | 161337.5 | 1 | UniProt (G0S652) UniProt (A0A366VY15) Pfam (PF07819) In PDB | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (Thermochaetoides thermophila) | |
2 | B | Green fluorescent protein,Complement decay-accelerating factor | polymer | 272 | 29894.2 | 1 | UniProt (P08174) In PDB | synthetic construct | |
3 | A | alpha-D-mannopyranose | non-polymer | 180.2 | 2 | Chemie (MAN) | |||
4 | A | 2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate | non-polymer | 303.2 | 1 | Chemie (05E) | |||
5 | A | 2-amino-2-deoxy-alpha-D-glucopyranose | non-polymer | 179.2 | 1 | Chemie (PA1) | |||
6 | A | [(2~{R})-1-octadecoxy-3-[oxidanyl-[(2~{R},3~{R},5~{S},6~{R})-2,3,4,5,6-pentakis(oxidanyl)cyclohexyl]oxy-phosphoryl]oxy-propan-2-yl] octadecanoate | non-polymer | 853.2 | 1 | Chemie (L9H) | |||
7 | A | PALMITIC ACID | non-polymer | 256.4 | 1 | Chemie (PLM) | |||
8 | A | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) | |||
9 | B | 2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate | non-polymer | 303.2 | 1 | Chemie (80Y) |
Sequence modifications
A: 2 - 1184 (UniProt: G0S652)
A: 1199 - 1433 (UniProt: A0A366VY15)
B: -267 - -5 (PDB: 8K9R)
B: -4 - 4 (UniProt: P08174)
PDB | External Database | Details |
---|---|---|
Met -1 | - | initiating methionine |
Gly 0 | - | expression tag |
Ser 1 | - | expression tag |
Asn 443 | His 443 | engineered mutation |
Gly 1185 | - | linker |
Thr 1186 | - | linker |
Leu 1187 | - | linker |
Glu 1188 | - | linker |
Val 1189 | - | linker |
Leu 1190 | - | linker |
Phe 1191 | - | linker |
Gln 1192 | - | linker |
Gly 1193 | - | linker |
Pro 1194 | - | linker |
Lys 1195 | - | linker |
Leu 1196 | - | linker |
Glu 1197 | - | linker |
Phe 1198 | - | linker |
PDB | External Database | Details |
---|---|---|
Ser 1345 | Trp 148 | engineered mutation |
Val 1363 | Ile 166 | engineered mutation |
Tyr 1365 | Gln 168 | engineered mutation |
Arg 1399 | Ile 202 | engineered mutation |
Ser 1434 | - | expression tag |
Ala 1435 | - | expression tag |
His 1436 | - | expression tag |
His 1437 | - | expression tag |
His 1438 | - | expression tag |
His 1439 | - | expression tag |
His 1440 | - | expression tag |
His 1441 | - | expression tag |
His 1442 | - | expression tag |
His 1443 | - | expression tag |
His 1444 | - | expression tag |
His 1445 | - | expression tag |
B: -4 - 4 (UniProt: P08174)
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 191231.7 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 2642.1 | |
All* | Total formula weight | 193873.8 |