8K8J

Cannabinoid Receptor 1 bound to Fenofibrate coupling MiniGsq and Nb35 Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	263	30652.6	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	97	10498.0	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	139	15271.9	1		Homo sapiens (human)
5	E (R)	Soluble cytochrome b562,Cannabinoid receptor 1	polymer	525	59685.4	1	UniProt (P0ABE7) UniProt (P21554) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,CB-R,CB1,CANN6
6	F (R)	Fenofibrate	non-polymer		360.8	1	Chemie (J3O)

Sequence modifications

A: 5 - 195 (UniProt: P63092)

PDB	External Database	Details
Met -9	-	initiating methionine
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Glu -1	-	expression tag
Asn 0	-	expression tag
Leu 1	-	expression tag
Tyr 2	-	expression tag
Phe 3	-	expression tag
Gln 4	-	expression tag
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict

A: 204 - 361 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 235	conflict
Asp 252	Ser 238	conflict
-	Asn 240	deletion
-	Met 241	deletion
-	Val 242	deletion
-	Ile 243	deletion
-	Arg 244	deletion
-	Glu 245	deletion
-	Asp 246	deletion
-	Asn 247	deletion
-	Gln 248	deletion
-	Thr 249	deletion
Ala 362	-	expression tag
Arg 363	-	expression tag
Arg 364	-	expression tag
Ile 365	-	expression tag
Phe 366	-	expression tag
Asn 367	-	expression tag
Asp 368	-	expression tag
Cys 369	-	expression tag
Lys 370	-	expression tag
Asp 371	-	expression tag
Ile 372	-	expression tag
Ile 373	-	expression tag
Leu 374	-	expression tag
Gln 375	-	expression tag
Met 376	-	expression tag
Asn 377	-	expression tag
Leu 378	-	expression tag
Arg 379	-	expression tag
Glu 380	-	expression tag
Tyr 381	-	expression tag
Asn 382	-	expression tag
Leu 383	-	expression tag
Val 384	-	expression tag

G: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Met -25	-	initiating methionine
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Asp -14	-	expression tag
Ser -13	-	expression tag
Leu -12	-	expression tag
Glu -11	-	expression tag
Phe -10	-	expression tag
Ile -9	-	expression tag
Ala -8	-	expression tag
Ser -7	-	expression tag
Lys -6	-	expression tag
Leu -5	-	expression tag
Ala -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 68	Cys 68	conflict

R: -43 - 61 (UniProt: P0ABE7)

PDB	External Database	Details
Met -92	-	initiating methionine
Lys -91	-	expression tag
Thr -90	-	expression tag
Ile -89	-	expression tag
Ile -88	-	expression tag
Ala -87	-	expression tag
Leu -86	-	expression tag
Ser -85	-	expression tag
Tyr -84	-	expression tag
Ile -83	-	expression tag
Phe -82	-	expression tag
Cys -81	-	expression tag
Leu -80	-	expression tag
Val -79	-	expression tag
Phe -78	-	expression tag
Ala -77	-	expression tag
Asp -76	-	expression tag
Tyr -75	-	expression tag
Lys -74	-	expression tag
Asp -73	-	expression tag
Asp -72	-	expression tag
Asp -71	-	expression tag
Asp -70	-	expression tag
Ala -69	-	expression tag
Lys -68	-	expression tag
Leu -67	-	expression tag
Gln -66	-	expression tag
Thr -65	-	expression tag
Met -64	-	expression tag
His -63	-	expression tag
His -62	-	expression tag
His -61	-	expression tag
His -60	-	expression tag
His -59	-	expression tag
His -58	-	expression tag
His -57	-	expression tag
His -56	-	expression tag
His -55	-	expression tag
His -54	-	expression tag
Glu -53	-	expression tag
Asn -52	-	expression tag
Leu -51	-	expression tag
Tyr -50	-	expression tag
Phe -49	-	expression tag
Gln -48	-	expression tag
Gly -47	-	expression tag
Gly -46	-	expression tag
Gly -45	-	expression tag
Arg -44	-	expression tag
Trp -37	Met 29	conflict
Ile 58	His 124	conflict
Leu 62	-	linker
Leu 63	-	linker
Glu 64	-	linker
Val 65	-	linker
Leu 66	-	linker
Phe 67	-	linker
Gln 68	-	linker
Gly 69	-	linker
Pro 70	-	linker

R: 71 - 425 (UniProt: P21554)

PDB	External Database	Details
Ile 210	Thr 210	conflict
Lys 273	Glu 273	conflict
Val 283	Thr 283	conflict
Glu 340	Arg 340	conflict
His 426	-	expression tag
His 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	153524.7
Non-Polymers*	Number of molecules	1
	Total formula weight	360.8
All*	Total formula weight	153885.6

*Water molecules are not included.