8K69

Cryo-EM structure of Oryza sativa HKT2;2/1 at 2.3 angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cation transporter HKT2;2	polymer	543	60773.2	2	UniProt (Q93XI5) Pfam (PF02386)	Oryza sativa subsp. indica (Rice)	OsHKT2;2,Cation transporter HKT2,OsHKT2,Po-OsHKT2
2	C, D, M, N (A, B)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	4	Chemie (Y01)
3	E, O (A, B)	Phosphatidylinositol	non-polymer		887.1	2	Chemie (T7X)
4	F, P (A, B)	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	2	Chemie (PTY)
5	G, Q (A, B)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
6	H, L (A, B)	1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		790.1	2	Chemie (PC1)
7	I, J, K, R, S... (A, B)	SODIUM ION	non-polymer		23.0	6	Chemie (NA)
8	U, V (A, B)	water	water		18.0	126	Chemie (HOH)

Sequence modifications

A, B: 1 - 530 (UniProt: Q93XI5)

PDB	External Database	Details
Met -12	-	initiating methionine
Ala -11	-	expression tag
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Val 167	Ala 167	conflict
Ala 337	Ser 337	conflict
Gln 420	Arg 420	conflict
Ile 430	Asn 430	conflict
Ser 431	Ala 431	conflict
Phe 438	Leu 438	conflict
Ile 461	Val 461	conflict
Ser 471	Thr 471	conflict
Phe 509	Ser 509	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	121546.5
Non-Polymers*	Number of molecules	18
	Total formula weight	7680.8
All*	Total formula weight	129227.3

*Water molecules are not included.