8K66

Cryo-EM structure of Oryza sativa HKT2;1 at 2.5 angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cation transporter HKT2;1	polymer	543	60822.3	2	UniProt (Q0D9S3) Pfam (PF02386)	Oryza sativa subsp. japonica (Rice)	OsHKT2;1,Cation transporter HKT1,OsHKT1,Ni-OsHKT1
2	C, D, L, M (A, B)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	4	Chemie (Y01)
3	E, N (A, B)	Phosphatidylinositol	non-polymer		887.1	2	Chemie (T7X)
4	F, O (A, B)	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	2	Chemie (PTY)
5	G, P (A, B)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
6	H, K (A, B)	1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		790.1	2	Chemie (PC1)
7	I, J, Q, R (A, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
8	S, T (A, B)	water	water		18.0	130	Chemie (HOH)

Sequence modifications

A, B: 1 - 530 (UniProt: Q0D9S3)

PDB	External Database	Details
Met -12	-	initiating methionine
Ala -11	-	expression tag
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	121644.6
Non-Polymers*	Number of molecules	16
	Total formula weight	7634.8
All*	Total formula weight	129279.4

*Water molecules are not included.