8K59

The cryo-EM map of TIC-TIEA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	DNA-directed RNA polymerase subunit beta	polymer	1340	150590.5	1	UniProt (P0A8V2) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560)	Escherichia coli K-12	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
2	B (D)	DNA-directed RNA polymerase subunit beta'	polymer	1363	150865.8	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Escherichia coli K-12	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
3	C (E)	DNA-directed RNA polymerase subunit omega	polymer	76	8649.7	1	UniProt (P0A800) Pfam (PF01192)	Escherichia coli K-12	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
4	D (F)	RNA polymerase sigma factor RpoD	polymer	523	60442.4	1	UniProt (P00579) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Escherichia coli K-12	Sigma-70
5	E, F (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	231	25612.1	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000)	Escherichia coli K-12	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
6	G (G)	15 kDa RNA polymerase-binding protein	polymer	129	14731.7	1	UniProt (P07879) Pfam (PF10789)	Escherichia phage T4	P15k
7	H (I)	DNA (61-MER)	polymer	61	18804.1	1		Escherichia coli K-12
8	I (H)	DNA (63-MER)	polymer	63	19356.4	1		Escherichia coli K-12
9	J (J)	RNA (5'-R(*AP*GP*GP*UP*A)-3')	polymer	5	1610.0	1		Escherichia coli K-12

Sequence modifications

A, B: 7 - 236 (UniProt: P0A7Z4)

PDB	External Database	Details
Thr 6	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	476274.8
All*	Total formula weight	476274.8

*Water molecules are not included.