8K07

Pseudouridine 5'-monophosphate glycosylase from Arabidopsis thaliana -- citrate bound K185A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Pseudouridine-5'-phosphate glycosidase	polymer	340	36124.0	3	UniProt (Q84K35) Pfam (PF04227)	Arabidopsis thaliana (thale cress)	pseudouridine 5'-monophosphate glycosylase,ribose 5'-phosphate adduct,PsiMP glycosidase,Pseudouridine monophosphate glycosidase
2	D, F, H (A, B, C)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
3	E, G, I (A, B, C)	CITRIC ACID	non-polymer		192.1	3	Chemie (CIT)
4	J, K, L (A, B, C)	water	water		18.0	809	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 330 (UniProt: Q84K35)

PDB	External Database	Details
Ala 185	Lys 185	engineered mutation
Leu 331	-	expression tag
Glu 332	-	expression tag
His 333	-	expression tag
His 334	-	expression tag
His 335	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	108372.0
Non-Polymers*	Number of molecules	6
	Total formula weight	741.2
All*	Total formula weight	109113.1

*Water molecules are not included.