8K06

Pseudouridine 5'-monophosphate glycosylase from Arabidopsis thaliana -- PSU, R5P bound K185A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Pseudouridine-5'-phosphate glycosidase	polymer	340	36124.0	3	UniProt (Q84K35) Pfam (PF04227) In PDB	Arabidopsis thaliana (thale cress)	PsiMP glycosidase,Pseudouridine monophosphate glycosidase
2	A, B, C	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
3	A, C	PSEUDOURIDINE-5'-MONOPHOSPHATE	non-polymer		324.2	2	Chemie (PSU)
4	B	5-O-phosphono-beta-D-ribofuranose	non-polymer		230.1	1	Chemie (RP5)
5		water	water		18.0	646	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 330 (UniProt: Q84K35)

PDB	External Database	Details
Ala 185	Lys 185	engineered mutation
Leu 331	-	expression tag
Glu 332	-	expression tag
His 333	-	expression tag
His 334	-	expression tag
His 335	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	108372.0
Non-Polymers*	Number of molecules	6
	Total formula weight	1043.3
All*	Total formula weight	109415.3

*Water molecules are not included.