8JXR
Structure of nanobody-bound DRD1_LSD complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | D(1A) dopamine receptor | polymer | 352 | 39187.8 | 1 | UniProt (P21728) Pfam (PF00001) In PDB | Homo sapiens (human) | Dopamine D1 receptor, DRD1 |
| 2 | B | NBA3 | polymer | 125 | 13494.0 | 1 | Homo sapiens | ||
| 3 | C | Maltose/maltodextrin-binding periplasmic protein,Immunoglobulin G-binding protein A,Immunoglobulin G-binding protein G | polymer | 559 | 60575.7 | 1 | UniProt (P0AEX9) UniProt (P38507) UniProt (P0A015) UniProt (P06654) Pfam (PF01547) Pfam (PF02216) Pfam (PF17573) Pfam (PF01378) In PDB | Escherichia coli O157:H7 | MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,IgG-binding protein A,Staphylococcal protein A,SpA,IgG-binding protein G |
| 4 | H | Fab 8D3 heavy chain | polymer | 253 | 27396.7 | 1 | Mus musculus | ||
| 5 | L | Fab 8D3 light chain | polymer | 239 | 26317.5 | 1 | Mus musculus | ||
| 6 | D | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | |||
| 7 | A | (8alpha)-N,N-diethyl-6-methyl-9,10-didehydroergoline-8-carboxamide | non-polymer | 323.4 | 1 | Chemie (7LD) |
Sequence modifications
A: 11 - 362 (UniProt: P21728)
C: 2 - 369 (UniProt: P0AEX9)
C: 371 - 409 (UniProt: P38507)
C: 419 - 467 (UniProt: P0A015)
C: 479 - 536 (UniProt: P06654)
| PDB | External Database | Details |
|---|---|---|
| Trp 112 | Leu 112 | engineered mutation |
| Ala 325 | Ser 325 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | cloning artifact |
| Ser -17 | - | cloning artifact |
| Ser -16 | - | cloning artifact |
| His -15 | - | cloning artifact |
| His -14 | - | cloning artifact |
| His -13 | - | cloning artifact |
| His -12 | - | cloning artifact |
| His -11 | - | cloning artifact |
| His -10 | - | cloning artifact |
| Ser -9 | - | cloning artifact |
| Ser -8 | - | cloning artifact |
| Gly -7 | - | cloning artifact |
| Leu -6 | - | cloning artifact |
| Val -5 | - | cloning artifact |
| Pro -4 | - | cloning artifact |
| Arg -3 | - | cloning artifact |
| Gly -2 | - | cloning artifact |
| Ser -1 | - | cloning artifact |
| His 0 | - | cloning artifact |
| Met 1 | - | cloning artifact |
| Gln 360 | Glu 385 | engineered mutation |
| Ala 363 | Lys 388 | engineered mutation |
| Phe 364 | Asp 389 | engineered mutation |
| Ile 367 | Thr 392 | engineered mutation |
| Leu 368 | Arg 393 | engineered mutation |
| Met 370 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Glu 404 | Asp 322 | conflict |
| His 405 | Ala 323 | conflict |
| Gly 410 | - | linker |
| Gly 411 | - | linker |
| Ser 412 | - | linker |
| Gly 413 | - | linker |
| Gly 414 | - | linker |
| Ala 415 | - | linker |
| Gly 416 | - | linker |
| Ser 417 | - | linker |
| Gly 418 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 468 | - | linker |
| Gly 469 | - | linker |
| Gly 470 | - | linker |
| Ser 471 | - | linker |
| Gly 472 | - | linker |
| Gly 473 | - | linker |
| Gly 474 | - | linker |
| Ser 475 | - | linker |
| Gly 476 | - | linker |
| Gly 477 | - | linker |
| Ser 478 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 537 | - | cloning artifact |
| Ser 538 | - | cloning artifact |
| Gly 539 | - | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 166971.8 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 323.4 | |
| All* | Total formula weight | 167637.5 |
