8JTR
Cryo-EM structure of GeoCas9-sgRNA binary complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR-associated endonuclease Cas9 | polymer | 1095 | 128171.0 | 1 | UniProt (A0A150MP45) Pfam (PF18470) Pfam (PF13395) Pfam (PF18541) In PDB | Geobacillus stearothermophilus | |
2 | B | sgRNA (139-bp) | polymer | 139 | 44697.4 | 1 | Geobacillus stearothermophilus |
Sequence modifications
A: 1 - 1087 (UniProt: A0A150MP45)
PDB | External Database | Details |
---|---|---|
Ala 219 | Thr 219 | conflict |
Ala 241 | Thr 241 | conflict |
Glu 353 | Gly 353 | conflict |
Ala 582 | His 582 | engineered mutation |
Leu 1088 | - | expression tag |
Glu 1089 | - | expression tag |
His 1090 | - | expression tag |
His 1091 | - | expression tag |
His 1092 | - | expression tag |
His 1093 | - | expression tag |
His 1094 | - | expression tag |
His 1095 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 172868.4 | |
All* | Total formula weight | 172868.4 |