8JTR

Cryo-EM structure of GeoCas9-sgRNA binary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR-associated endonuclease Cas9	polymer	1095	128171.0	1	UniProt (A0A150MP45)	Geobacillus stearothermophilus
2	B (B)	sgRNA (139-bp)	polymer	139	44697.4	1		Geobacillus stearothermophilus

Sequence modifications

A: 1 - 1087 (UniProt: A0A150MP45)

PDB	External Database	Details
Ala 219	Thr 219	conflict
Ala 241	Thr 241	conflict
Glu 353	Gly 353	conflict
Ala 582	His 582	engineered mutation
Leu 1088	-	expression tag
Glu 1089	-	expression tag
His 1090	-	expression tag
His 1091	-	expression tag
His 1092	-	expression tag
His 1093	-	expression tag
His 1094	-	expression tag
His 1095	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	172868.4
All*	Total formula weight	172868.4

*Water molecules are not included.