8JSR

Cryo-EM structure of the anamorelin-bound ghrelin receptor and Gq complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Growth hormone secretagogue receptor type 1	polymer	523	58827.0	1	UniProt (Q92847) Pfam (PF00001)	Homo sapiens (human)	GHS-R,GH-releasing peptide receptor,GHRP,Ghrelin receptor
2	B (A)	Engineered G-alpha-q	polymer	361	41724.4	1		Homo sapiens (human)
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	388	42282.2	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
4	D (C)	scFv16	polymer	259	27784.9	1		Mus musculus
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (N)	Nb35	polymer	129	14016.6	1		Lama glama
7	G (R)	Anamorelin	non-polymer		546.7	1	Chemie (UYI)

Sequence modifications

R: 2 - 366 (UniProt: Q92847)

PDB	External Database	Details
Val 367	-	expression tag
Phe 368	-	expression tag
Thr 369	-	expression tag
Leu 370	-	expression tag
Glu 371	-	expression tag
Asp 372	-	expression tag
Phe 373	-	expression tag
Val 374	-	expression tag
Gly 375	-	expression tag
Asp 376	-	expression tag
Trp 377	-	expression tag
Glu 378	-	expression tag
Gln 379	-	expression tag
Thr 380	-	expression tag
Ala 381	-	expression tag
Ala 382	-	expression tag
Tyr 383	-	expression tag
Asn 384	-	expression tag
Leu 385	-	expression tag
Asp 386	-	expression tag
Gln 387	-	expression tag
Val 388	-	expression tag
Leu 389	-	expression tag
Glu 390	-	expression tag
Gln 391	-	expression tag
Gly 392	-	expression tag
Gly 393	-	expression tag
Val 394	-	expression tag
Ser 395	-	expression tag
Ser 396	-	expression tag
Leu 397	-	expression tag
Leu 398	-	expression tag
Gln 399	-	expression tag
Asn 400	-	expression tag
Leu 401	-	expression tag
Ala 402	-	expression tag
Val 403	-	expression tag
Ser 404	-	expression tag
Val 405	-	expression tag
Thr 406	-	expression tag
Pro 407	-	expression tag
Ile 408	-	expression tag
Gln 409	-	expression tag
Arg 410	-	expression tag
Ile 411	-	expression tag
Val 412	-	expression tag
Arg 413	-	expression tag
Ser 414	-	expression tag
Gly 415	-	expression tag
Glu 416	-	expression tag
Asn 417	-	expression tag
Ala 418	-	expression tag
Leu 419	-	expression tag
Lys 420	-	expression tag
Ile 421	-	expression tag
Asp 422	-	expression tag
Ile 423	-	expression tag
His 424	-	expression tag
Val 425	-	expression tag
Ile 426	-	expression tag
Ile 427	-	expression tag
Pro 428	-	expression tag
Tyr 429	-	expression tag
Glu 430	-	expression tag
Gly 431	-	expression tag
Leu 432	-	expression tag
Ser 433	-	expression tag
Ala 434	-	expression tag
Asp 435	-	expression tag
Gln 436	-	expression tag
Met 437	-	expression tag
Ala 438	-	expression tag
Gln 439	-	expression tag
Ile 440	-	expression tag
Glu 441	-	expression tag
Glu 442	-	expression tag
Val 443	-	expression tag
Phe 444	-	expression tag
Lys 445	-	expression tag
Val 446	-	expression tag
Val 447	-	expression tag
Tyr 448	-	expression tag
Pro 449	-	expression tag
Val 450	-	expression tag
Asp 451	-	expression tag
Asp 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
Phe 455	-	expression tag
Lys 456	-	expression tag
Val 457	-	expression tag
Ile 458	-	expression tag
Leu 459	-	expression tag
Pro 460	-	expression tag
Tyr 461	-	expression tag
Gly 462	-	expression tag
Thr 463	-	expression tag
Leu 464	-	expression tag
Val 465	-	expression tag
Ile 466	-	expression tag
Asp 467	-	expression tag
Gly 468	-	expression tag
Val 469	-	expression tag
Thr 470	-	expression tag
Pro 471	-	expression tag
Asn 472	-	expression tag
Met 473	-	expression tag
Leu 474	-	expression tag
Asn 475	-	expression tag
Tyr 476	-	expression tag
Phe 477	-	expression tag
Gly 478	-	expression tag
Arg 479	-	expression tag
Pro 480	-	expression tag
Tyr 481	-	expression tag
Glu 482	-	expression tag
Gly 483	-	expression tag
Ile 484	-	expression tag
Ala 485	-	expression tag
Val 486	-	expression tag
Phe 487	-	expression tag
Asp 488	-	expression tag
Gly 489	-	expression tag
Lys 490	-	expression tag
Lys 491	-	expression tag
Ile 492	-	expression tag
Thr 493	-	expression tag
Val 494	-	expression tag
Thr 495	-	expression tag
Gly 496	-	expression tag
Thr 497	-	expression tag
Leu 498	-	expression tag
Trp 499	-	expression tag
Asn 500	-	expression tag
Gly 501	-	expression tag
Asn 502	-	expression tag
Lys 503	-	expression tag
Ile 504	-	expression tag
Ile 505	-	expression tag
Asp 506	-	expression tag
Glu 507	-	expression tag
Arg 508	-	expression tag
Leu 509	-	expression tag
Ile 510	-	expression tag
Thr 511	-	expression tag
Pro 512	-	expression tag
Asp 513	-	expression tag
Gly 514	-	expression tag
Ser 515	-	expression tag
Met 516	-	expression tag
Leu 517	-	expression tag
Phe 518	-	expression tag
Arg 519	-	expression tag
Val 520	-	expression tag
Thr 521	-	expression tag
Ile 522	-	expression tag
Asn 523	-	expression tag
Ser 524	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -21	-	initiating methionine
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	linker
Ser 342	-	linker
Ser 343	-	linker
Gly 344	-	linker
Gly 345	-	linker
Gly 346	-	linker
Gly 347	-	linker
Ser 348	-	linker
Gly 349	-	linker
Gly 350	-	linker
Gly 351	-	linker
Gly 352	-	linker
Ser 353	-	linker
Ser 354	-	linker
Gly 355	-	linker

B: 356 - 366 (PDB: 8JSR)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	192496.2
Non-Polymers*	Number of molecules	1
	Total formula weight	546.7
All*	Total formula weight	193042.9

*Water molecules are not included.