8JQQ

Protocatecuate hydroxylase from Xylophilus ampelinus C347T mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	4-hydroxybenzoate 3-monooxygenase (NAD(P)H)	polymer	411	45529.7	2	UniProt (A0A978C2P2) UniProt (by SIFTS) (A0AAJ6N6I3)	Xylophilus ampelinus
2	C, E (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	D (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	F, G (A, B)	water	water		18.0	139	Chemie (HOH)

Sequence modifications

A, B: 2 - 391 (UniProt: A0A978C2P2)

PDB	External Database	Details
Met -19	-	initiating methionine
Gln -18	-	expression tag
Leu -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Thr 347	Cys 350	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	91059.4
Non-Polymers*	Number of molecules	3
	Total formula weight	1611.2
All*	Total formula weight	92670.6

*Water molecules are not included.