8JOZ

Crystal structure of CmoM from E. coli complexed with sinefungin and cellularly expressed tRNA Ser

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	tRNA 5-carboxymethoxyuridine methyltransferase	polymer	282	32199.9	1	UniProt (P36566) Pfam (PF13847)	Escherichia coli K-12	cmo5U methyltransferase
2	B (B)	tRNA Ser (88-MER)	polymer	88	28648.3	1		Escherichia coli K-12
3	C (A)	SINEFUNGIN	non-polymer		381.4	1	Chemie (SFG)
4	D, E, F, G, H... (B)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
5	K, L (A, B)	water	water		18.0	351	Chemie (HOH)

Sequence modifications

A: 2 - 261 (UniProt: P36566)

PDB	External Database	Details
Met -15	-	initiating methionine
Arg -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Thr -5	-	expression tag
Asp -4	-	expression tag
Pro -3	-	expression tag
Ala -2	-	expression tag
Leu -1	-	expression tag
Arg 0	-	expression tag
Ala 1	-	expression tag
Gly 262	-	expression tag
Leu 263	-	expression tag
Cys 264	-	expression tag
Gly 265	-	expression tag
Arg 266	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60848.2
Non-Polymers*	Number of molecules	8
	Total formula weight	551.5
All*	Total formula weight	61399.7

*Water molecules are not included.